| Creator |
-
Kyme, Pierre
-
Thoennissen, Nils H.
-
Tseng, Ching Wen
-
Thoennissen, Gabriela B.
-
Wolf, Andrea J.
-
Shimada, Kenichi
-
Krug, Utz O.
-
Lee, Kunik
-
Muller-Tidow, Carsten
-
Berdel, Wolfgang E.
-
Hardy, W. David
-
Gombart, Adrian F.
-
Koeffler, H. Phillip
-
Liu, George Y.
|
|---|
| Abstract |
- The myeloid-specific transcription factor, CCAAT/enhancer-binding protein epsilon (C/EBPε) is a critical mediator of myelopoiesis. Mutation of this gene is responsible for neutrophil-specific granule deficiency in humans, a condition that confers susceptibility to Staphylococcus aureus infection. We found that C/EBPε-deficient mice are severely affected by infection with S. aureus, and C/EBPε deficiency in neutrophils contributes to the infectious phenotype. Conversely, exposure to the epigenetic modulator nicotinamide (vitamin B3) increased expression of C/EBPε in WT myeloid cells. Further, nicotinamide increased the activity of C/EBPε and select downstream antimicrobial targets, particularly in neutrophils. In a systemic murine infection model as well as in murine and human peripheral blood, nicotinamide enhanced killing of S. aureus by up to 1,000 fold but had no effect when administered to either C/EBPε-deficient mice or mice depleted of neutrophils. Nicotinamide was efficacious in both prophylactic and therapeutic settings. Our findings suggest that C/EBPε is an important target to boost killing of bacteria by the innate immune system.
|
|---|
| Resource Type |
|
|---|
| DOI |
|
|---|
| Date Available |
|
|---|
| Date Issued |
|
|---|
| Citation |
- Kyme, P., Berdel, W. E., Hardy, W. D., Gombart, A. F., Koeffler, H. P., Liu, G. Y., . . . Müller-Tidow, C. (2012). C/EBPε mediates nicotinamide-enhanced clearance of staphylococcus aureus in mice. The Journal of Clinical Investigation, 122(9), 3316.
|
|---|
| Journal Title |
|
|---|
| Journal Volume |
|
|---|
| Journal Issue/Number |
|
|---|
| Academic Affiliation |
|
|---|
| Keyword |
|
|---|
| Rights Statement |
|
|---|
| Funding Statement (additional comments about funding) |
- This work was supported by a Burroughs-Wellcome Career Award (to G.Y. Liu); grants from the US NIH to G.Y. Liu (R01 AI074832), H.P. Koeffler (R01 CA026038-30; U54 CA143930-01), and A.F. Gombart (R01 AI065604-05); and the A*STAR Investigator grant (to H.P. Koeffler). N.H. Thoennissen was supported by the Deutsche Forschungsgemeinschaft, Bonn, Germany (TH 1438/1-1).
|
|---|
| Publisher |
|
|---|
| Peer Reviewed |
|
|---|
| Language |
|
|---|
| Replaces |
|
|---|
| Additional Information |
- description.provenance : Approved for entry into archive by Deborah Campbell(deborah.campbell@oregonstate.edu) on 2013-02-11T23:00:38Z (GMT) No. of bitstreams: 1
GombartAdrianFBiochemistryBiophysicsCEBPMediatesNicotinamide.pdf: 1948494 bytes, checksum: 827645372e11cbb311be6002a268c43f (MD5)
- description.provenance : Submitted by Deborah Campbell (deborah.campbell@oregonstate.edu) on 2013-02-11T23:00:05Z
No. of bitstreams: 1
GombartAdrianFBiochemistryBiophysicsCEBPMediatesNicotinamide.pdf: 1948494 bytes, checksum: 827645372e11cbb311be6002a268c43f (MD5)
- description.provenance : Made available in DSpace on 2013-02-11T23:00:38Z (GMT). No. of bitstreams: 1
GombartAdrianFBiochemistryBiophysicsCEBPMediatesNicotinamide.pdf: 1948494 bytes, checksum: 827645372e11cbb311be6002a268c43f (MD5)
Previous issue date: 2012-09
|
|---|