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Increase in cellular triacylglycerol content and emergence of large ER-associated lipid droplets in the absence of CDP-DG synthase function

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  • Excess fatty acids and sterols are stored as triacylglycerols and sterol esters in specialized cellular organelles, called lipid droplets. Understanding what determines the cellular amount of neutral lipids and their packaging into lipid droplets is of fundamental and applied interest. Using two species of fission yeast, we show that cycling cells deficient in the function of the ER-resident CDP-DG synthase Cds1 exhibit markedly increased triacylglycerol content and assemble large lipid droplets closely associated with the ER membranes. We demonstrate that these unusual structures recruit the triacylglycerol synthesis machinery and grow by expansion rather than by fusion. Our results suggest that interfering with the CDPDG route of phosphatidic acid utilization rewires cellular metabolism to adopt a triacylglycerol-rich lifestyle reliant on the Kennedy pathway.
  • This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by the American Society for Cell Biology. The published article can be found at: http://www.molbiolcell.org/.
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  • He, Y., Yam, C., Pomraning, K., Chin, J. S. R., Yew, J. Y., Freitag, M., & Oliferenko, S. (2014). Increase in cellular triacylglycerol content and emergence of large ER-associated lipid droplets in the absence of CDP-DG synthase function. Molecular Biology of the Cell, 25(25), 4083-4095. doi:10.1091/mbc.E14-03-0832
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  • 25
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  • 25
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  • The lipid profile data were acquired at the Kansas Lipidomics Research Center, where instrument acquisition and method was supported by National Science Foundation Grants MCB 0455318, MCB 0920663, and DBI 0521587, Kansas INBRE (National Institutes of Health Grant P20 RR16475 from the INBRE program of the National Center for Research Resources), National Science Foundation EPSCoR Grant EPS-0236913, the Kansas Technology Enterprise Corporation, and Kansas State University. This work was supported by the Singapore Millennium Foundation to S.O., the Singapore National Research Foundation (RF2010-06) to J.Y., and start-up funds from the Oregon State University Computational and Genome Biology Initiative to M.F.
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