Article

 

ZabriskieMarkPharmacyStandaloneAminopeptidasePepN(SupplementaryMaterial).pdf Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/articles/nv935482w

Descriptions

Attribute NameValues
Creator
Abstract
  • The peptidyl nucleoside blasticidin S (BS) isolated from Streptomyces griseochromogenes was the first non-mercurial fungicide used on a large scale to prevent rice blast. In the biosynthesis of BS, leucylblasticidin S (LBS) was suggested as the penultimate metabolite with 20-fold less inhibitory activity than the final product BS. Incomplete conversion of LBS to BS at a variable efficiency ranging from 10% to 90% was observed either in the native strain S. griseochromogenes or a heterologous producer Streptomyces lividans WJ2. In this study, we determined that maturation of BS from LBS is not a spontaneous process but is governed by a standalone peptidase PepN, which hydrolyzes LBS in a pH-sensitive way with most appropriate of pH 7~8 but is inactive when the pH is below 5 or above 10. PepN1 and PepN2, two neighboring PepN homologs from Streptomyces lividans were purified in E. coli but displayed ca.100-fold difference in LBS hydrolytic activity. Overexpression of pepN1 in WJ2 enhanced BS yield by 100% and lowered the ratio of LBS to BS from 2:1 to 2:3. This work presents the expansion of the biological role for PepN in antibiotic maturation and the first report of hydrolysis of beta amide linkage by this conserved enzyme.
Rights Statement
Additional Information
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2016-01-13T14:55:20Z (GMT) No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) ZabriskieMarkPharmacyStandaloneAminopeptidasePepN.pdf: 900661 bytes, checksum: 98e039803ce628ce51d7a34821f948a4 (MD5) ZabriskieMarkPharmacyStandaloneAminopeptidasePepN(SupplementaryMaterial).pdf: 965179 bytes, checksum: ce523e21b6b9d3722483df3f2dd99be1 (MD5)
  • description.provenance : Submitted by Patricia Black (patricia.black@oregonstate.edu) on 2016-01-13T14:54:56Z No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) ZabriskieMarkPharmacyStandaloneAminopeptidasePepN.pdf: 900661 bytes, checksum: 98e039803ce628ce51d7a34821f948a4 (MD5) ZabriskieMarkPharmacyStandaloneAminopeptidasePepN(SupplementaryMaterial).pdf: 965179 bytes, checksum: ce523e21b6b9d3722483df3f2dd99be1 (MD5)
  • description.provenance : Made available in DSpace on 2016-01-13T14:55:20Z (GMT). No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) ZabriskieMarkPharmacyStandaloneAminopeptidasePepN.pdf: 900661 bytes, checksum: 98e039803ce628ce51d7a34821f948a4 (MD5) ZabriskieMarkPharmacyStandaloneAminopeptidasePepN(SupplementaryMaterial).pdf: 965179 bytes, checksum: ce523e21b6b9d3722483df3f2dd99be1 (MD5) Previous issue date: 2015-12-01

Relationships

Parents:

Items