Identification of quantitative trait loci (QTL) for resistance to Fusarium crown rot (Fusarium pseudograminearum) in multiple assay environments in the Pacific Northwestern US

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  • Fusarium crown rot (FCR), caused by and , reduces wheat ( L.) yields in the Pacific Northwest (PNW) of the US by as much as 35%. Resistance to FCR has not yet been discovered in currently grown PNW wheat cultivars. Several significant quantitative trait loci (QTL) for FCR resistance have been documented on chromosomes 1A, 1D, 2B, 3B, and 4B in resistant Australian cultivars. Our objective was to identify QTL and tightly linked SSR markers for FCR resistance in the partially resistant Australian spring wheat cultivar Sunco using PNW isolates of in greenhouse and field based screening nurseries. A second objective was to compare heritabilities of FCR resistance in multiple types of disease assaying environments (seedling, terrace, and field) using multiple disease rating methods. Two recombinant inbred line (RIL) mapping populations were derived from crosses between Sunco and PNW spring wheat cultivars Macon and Otis. The Sunco/Macon population comprised 219 F₆:F₇ lines and the Sunco/Otis population comprised 151 F₅:F₆ lines. Plants were inoculated with a single PNW isolate (006-13) in growth room (seedling), outdoor terrace (adult) and field (adult) assays conducted from 2008 through 2010. Crown and lower stem tissues of seedling and adult plants were rated for disease severity on several different scales, but mainly on a numeric scale from 0 to 10 where 0 = no discoloration and 10 = severe disease. Significant QTL were identified on chromosomes 2B, 3B, 4B, 4D, and 7A with LOD scores ranging from 3 to 22. The most significant and consistent QTL across screening environments was located on chromosome 3BL, inherited from the PNW cultivars Macon and Otis, with maximum LOD scores of 22 and 9 explaining 36 and 23% of the variation, respectively for the Sunco/Macon and Sunco/Otis populations. The SSR markers and flank these QTL and are being validated for use in marker-assisted selection for FCR resistance. This is the first report of QTL associated with FCR resistance in the US.
  • Keywords: Root, Winter wheat, Management, Barley, Markers, Map, Seedling resistance, Triticum aestivum L, Graminearum group-1, Foot rot
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  • Poole, G. J., Smiley, R. W., Paulitz, T. C., Walker, C. A., Carter, A. H., See, D. R., & Garland-Campbell, K. (2012). Identification of quantitative trait loci (QTL) for resistance to fusarium crown rot ( fusarium pseudograminearum ) in multiple assay environments in the pacific northwestern US. Theoretical and Applied Genetics, 125(1), 91-107. doi: 10.1007/s00122-012-1818-6
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  • 125
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  • 1
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  • This research was funded by the Washington Grains Commission Project 7345 entitled ‘‘The development of wheat varieties resistant or tolerant to Fusarium crown rot’’. Additional funding was obtained from Washington Grains Commission projects 5345 and 6345, and USDA-ARS (in house) projects 5348-21220-003-00D and 5348-22000-013-00. Funding to pay the Open Access publication charges for this article was provided by the USDA-ARS. Open Access This article
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