Mechanistic insight into the conserved allosteric regulation of periplasmic proteolysis by the signaling molecule cyclic-di-GMP Public Deposited

http://ir.library.oregonstate.edu/concern/articles/qj72p9179

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  • Stable surface adhesion of cells is one of the early pivotal steps in bacterial biofilm formation, a prevalent adaptation strategy in response to changing environments. In Pseudomonas fluorescens, this process is regulated by the Lap system and the second messenger cyclic-di-GMP. High cytoplasmic levels of cyclic-di-GMP activate the transmembrane receptor LapD that in turn recruits the periplasmic protease LapG, preventing it from cleaving a cell surface-bound adhesin, thereby promoting cell adhesion. In this study, we elucidate the molecular basis of LapG regulation by LapD and reveal a remarkably sensitive switching mechanism that is controlled by LapD's HAMP domain. LapD appears to act as a coincidence detector, whereby a weak interaction of LapG with LapD transmits a transient outside-in signal that is reinforced only when cyclic-di-GMP levels increase. Given the conservation of key elements of this receptor system in many bacterial species, the results are broadly relevant for cyclic-di-GMP- and HAMP domain-regulated transmembrane signaling.
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  • Chatterjee, D., Cooley, R. B., Boyd, C. D., Mehl, R. A., O'Toole, G. A., & Sondermann, H. (2014). Mechanistic insight into the conserved allosteric regulation of periplasmic proteolysis by the signaling molecule cyclic-di-GMP. eLife, 3, e03650. doi:10.7554/eLife.03650
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  • description.provenance : Made available in DSpace on 2014-10-08T22:09:01Z (GMT). No. of bitstreams: 2 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) MehlRyanBiochemBiophysMechanisticInsight.pdf: 5389042 bytes, checksum: 13918fdbf62074dde7659c54e1b8b8bf (MD5) Previous issue date: 2014-09-02
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  • description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2014-10-08T22:09:01Z (GMT) No. of bitstreams: 2 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) MehlRyanBiochemBiophysMechanisticInsight.pdf: 5389042 bytes, checksum: 13918fdbf62074dde7659c54e1b8b8bf (MD5)

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