Enhanced resistance in Theobroma cacao against oomycete and fungal pathogens by secretion of phosphatidylinositol-3-phosphate-binding proteins Public Deposited

http://ir.library.oregonstate.edu/concern/articles/r494vm94t

To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The article is copyrighted by Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd. and published by Wiley Open Access. It can be found at:  http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291467-7652

Supporting information is available online at:  http://onlinelibrary.wiley.com/doi/10.1111/pbi.12436/abstract

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • The internalization of some oomycete and fungal pathogen effectors into host plant cells has been reported to be blocked by proteins that bind to the effectors' cell entry receptor, phosphatidylinositol-3-phosphate (PI3P). This finding suggested a novel strategy for disease control by engineering plants to secrete PI3P-binding proteins. In this study, we tested this strategy using the chocolate tree Theobroma cacao. Transient expression and secretion of four different PI3P-binding proteins in detached leaves of T. cacao greatly reduced infection by two oomycete pathogens, Phytophthora tropicalis and Phytophthora palmivora, which cause black pod disease. Lesion size and pathogen growth were reduced by up to 85%. Resistance was not conferred by proteins lacking a secretory leader, by proteins with mutations in their PI3P-binding site, or by a secreted PI4P-binding protein. Stably transformed, transgenic T. cacao plants expressing two different PI3P-binding proteins showed substantially enhanced resistance to both P. tropicalis and P. palmivora, as well as to the fungal pathogen Colletotrichum theobromicola. These results demonstrate that secretion of PI3P-binding proteins is an effective way to increase disease resistance in T. cacao, and potentially in other plants, against a broad spectrum of pathogens.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Helliwell, E. E., Vega‐Arreguín, J., Shi, Z., Bailey, B., Xiao, S., Maximova, S. N., ... & Guiltinan, M. J. (2016). Enhanced resistance in Theobroma cacao against oomycete and fungal pathogens by secretion of phosphatidylinositol‐3‐phosphate‐binding proteins. Plant Biotechnology Journal, 14(3), 875-886. doi:10.1111/pbi.12436
Series
Keyword
Rights Statement
Funding Statement (additional comments about funding)
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2016-04-05T14:09:34Z (GMT). No. of bitstreams: 1 HelliwellEnhancedResistanceTheobroma.pdf: 1387582 bytes, checksum: ae2a9fe8fa13e9e4b23de2037a5179f4 (MD5) Previous issue date: 2016-03
  • description.provenance : Submitted by Patricia Black (patricia.black@oregonstate.edu) on 2016-04-05T14:09:01Z No. of bitstreams: 1 HelliwellEnhancedResistanceTheobroma.pdf: 1387582 bytes, checksum: ae2a9fe8fa13e9e4b23de2037a5179f4 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2016-04-05T14:09:34Z (GMT) No. of bitstreams: 1 HelliwellEnhancedResistanceTheobroma.pdf: 1387582 bytes, checksum: ae2a9fe8fa13e9e4b23de2037a5179f4 (MD5)

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items