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Enhanced resistance in Theobroma cacao against oomycete and fungal pathogens by secretion of phosphatidylinositol-3-phosphate-binding proteins

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https://ir.library.oregonstate.edu/concern/articles/r494vm94t

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  • The internalization of some oomycete and fungal pathogen effectors into host plant cells has been reported to be blocked by proteins that bind to the effectors' cell entry receptor, phosphatidylinositol-3-phosphate (PI3P). This finding suggested a novel strategy for disease control by engineering plants to secrete PI3P-binding proteins. In this study, we tested this strategy using the chocolate tree Theobroma cacao. Transient expression and secretion of four different PI3P-binding proteins in detached leaves of T. cacao greatly reduced infection by two oomycete pathogens, Phytophthora tropicalis and Phytophthora palmivora, which cause black pod disease. Lesion size and pathogen growth were reduced by up to 85%. Resistance was not conferred by proteins lacking a secretory leader, by proteins with mutations in their PI3P-binding site, or by a secreted PI4P-binding protein. Stably transformed, transgenic T. cacao plants expressing two different PI3P-binding proteins showed substantially enhanced resistance to both P. tropicalis and P. palmivora, as well as to the fungal pathogen Colletotrichum theobromicola. These results demonstrate that secretion of PI3P-binding proteins is an effective way to increase disease resistance in T. cacao, and potentially in other plants, against a broad spectrum of pathogens.
  • This is the publisher’s final pdf. The article is copyrighted by Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd. and published by Wiley Open Access. It can be found at: http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291467-7652
  • Keywords: phosphatidylinositol-3-phosphate-binding protein, oomycetes, disease resistance, fungi, Theobroma cacao, effectors
  • Keywords: phosphatidylinositol-3-phosphate-binding protein, oomycetes, disease resistance, fungi, Theobroma cacao, effectors
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  • Helliwell, E. E., Vega‐Arreguín, J., Shi, Z., Bailey, B., Xiao, S., Maximova, S. N., ... & Guiltinan, M. J. (2016). Enhanced resistance in Theobroma cacao against oomycete and fungal pathogens by secretion of phosphatidylinositol‐3‐phosphate‐binding proteins. Plant Biotechnology Journal, 14(3), 875-886. doi:10.1111/pbi.12436
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  • 14
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  • 3
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  • This work was supported in part by The Pennsylvania State University, College of Agricultural Sciences, The Huck Institutes of Life Sciences, the American Research Institute Penn State Endowed Program in the Molecular Biology of Cacao and grants from the National Science Foundation BREAD Program (IOS-0965353) to BT, MG, SM and SX, and to BT from the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number (2011-68004-30104).
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