Article

 

An Interspecies Signaling System Mediated by Fusaric Acid Has Parallel Effects on Antifungal Metabolite Production by Pseudomonas protegens Strain Pf-5 and Antibiosis of Fusarium spp. Public Deposited

Analytics
Default
https://ir.library.oregonstate.edu/concern/articles/r494vm98x

To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The published article is copyrighted by American Society for Microbiology and can be found at:  http://aem.asm.org/

Descriptions

Attribute NameValues
Creator
Abstract
  • Pseudomonas protegens strain Pf-5 is a rhizosphere bacterium that suppresses soilborne plant diseases and produces at least seven different secondary metabolites with antifungal properties. We derived mutants of Pf-5 with single and multiple mutations in biosynthesis genes for seven antifungal metabolites: 2,4-diacetylphoroglucinol (DAPG), pyrrolnitrin, pyoluteorin, hydrogen cyanide, rhizoxin, orfamide A, and toxoflavin. These mutants were tested for inhibition of the pathogens Fusarium verticillioides and Fusarium oxysporum f. sp. pisi. Rhizoxin, pyrrolnitrin, and DAPG were found to be primarily responsible for fungal antagonism by Pf-5. Previously, other workers showed that the mycotoxin fusaric acid, which is produced by many Fusarium species, including F. verticillioides, inhibited the production of DAPG by Pseudomonas spp. In this study, amendment of culture media with fusaric acid decreased DAPG production, increased pyoluteorin production, and had no consistent influence on pyrrolnitrin or orfamide A production by Pf-5. Fusaric acid also altered the transcription of biosynthetic genes, indicating that the mycotoxin influenced antibiotic production by Pf-5 at the transcriptional level. Addition of fusaric acid to the culture medium reduced antibiosis of F. verticillioides by Pf-5 and derivative strains that produce DAPG but had no effect on antibiosis by Pf-5 derivatives that suppressed F. verticillioides due to pyrrolnitrin or rhizoxin production. Our results demonstrated the importance of three compounds, rhizoxin, pyrrolnitrin, and DAPG, in suppression of Fusarium spp. by Pf-5 and confirmed that an interspecies signaling system mediated by fusaric acid had parallel effects on antifungal metabolite production and antibiosis by the bacterial biological control organism.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Quecine, M. C., Kidarsa, T. A., Goebel, N. C., Shaffer, B. T., Henkels, M. D., Zabriskie, T. M., & Loper, J. E. (2016). An inter-species signaling system mediated by fusaric acid has parallel effects on antifungal metabolite production by Pseudomonas protegens Pf-5 and antibiosis of Fusarium spp. Applied and Environmental Microbiology, 82(5), 1372-1382. doi:10.1128/AEM.02574-15
Journal Title
Journal Volume
  • 82
Journal Issue/Number
  • 5
Rights Statement
Funding Statement (additional comments about funding)
  • USDA| National Institute of Food and Agriculture (NIFA) provided funding to Joyce E. Loper under grant number 2006-35319-17427. USDA | National Institute of Food and Agriculture (NIFA) provided funding to T. Mark Zabriskie and Joyce E. Loper under grant number 2011-67019-30192. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) provided funding to Maria Carolina Quecine under grant number 2005-53748-6.
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2016-04-25T16:40:58Z (GMT) No. of bitstreams: 2 QuecineInterspeciesSignalingSystem.pdf: 993397 bytes, checksum: 3b6f411219ee517f063371bf0b38f12e (MD5) QuecineInterspeciesSignalingSystemSupplement.pdf: 136639 bytes, checksum: 8bf154895fab4f010da86a07fa6ac4a2 (MD5)
  • description.provenance : Submitted by Patricia Black (patricia.black@oregonstate.edu) on 2016-04-25T16:40:30Z No. of bitstreams: 2 QuecineInterspeciesSignalingSystem.pdf: 993397 bytes, checksum: 3b6f411219ee517f063371bf0b38f12e (MD5) QuecineInterspeciesSignalingSystemSupplement.pdf: 136639 bytes, checksum: 8bf154895fab4f010da86a07fa6ac4a2 (MD5)
  • description.provenance : Made available in DSpace on 2016-04-25T16:40:58Z (GMT). No. of bitstreams: 2 QuecineInterspeciesSignalingSystem.pdf: 993397 bytes, checksum: 3b6f411219ee517f063371bf0b38f12e (MD5) QuecineInterspeciesSignalingSystemSupplement.pdf: 136639 bytes, checksum: 8bf154895fab4f010da86a07fa6ac4a2 (MD5) Previous issue date: 2016-03

Relationships

Parents:

This work has no parents.

Items

Thumbnail Title Date Uploaded Visibility Actions
7w62fb296?file=thumbnail QuecineInterspeciesSignalingSystem.pdf 07/26/2017 Public
8336h3943?file=thumbnail QuecineInterspeciesSignalingSystemSupplement.pdf 07/26/2017 Public