Genome Sequencing and Transposon Mutagenesis of Burkholderia seminalis TC3.4.2R3 Identify Genes Contributing to Suppression of Orchid Necrosis Caused by B-gladioli Public Deposited

http://ir.library.oregonstate.edu/concern/articles/r781wh61h

To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The article is published by APS PRESS in cooperation with the International Society for Molecular Plant-Microbe Interactions. The published article is copyrighted the American Phytopathological Society and It can be found at:  http://apsjournals.apsnet.org/loi/mpmi

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • From a screen of 36 plant-associated strains of Burkholderia spp., we identified 24 strains that suppressed leaf and pseudobulb necrosis of orchid caused by B. gladioli. To gain insights into the mechanisms of disease suppression, we generated a draft genome sequence from one suppressive strain, TC3.4.2R3. The genome is an estimated 7.67 megabases in size, with three replicons, two chromosomes, and the plasmid pC3. Using a combination of multilocus sequence analysis and phylogenomics, we identified TC3.4.2R3 as B. seminalis, a species within the Burkholderia cepacia complex that includes opportunistic human pathogens and environmental strains. We generated and screened a library of 3,840 transposon mutants of strain TC3.4.2R3 on orchid leaves to identify genes contributing to plant disease suppression. Twelve mutants deficient in suppression of leaf necrosis were selected and the transposon insertions were mapped to eight loci. One gene is in a wcb cluster that is related to synthesis of extracellular polysaccharide, a key determinant in bacterial-host interactions in other systems, and the other seven are highly conserved among Burkholderia spp. The fundamental information developed in this study will serve as a resource for future research aiming to identify mechanisms contributing to biological control.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Araújo, W. L., Creason, A. L., Mano, E. T., Camargo-Neves, A. A., Minami, S. N., Chang, J. H., & Loper, J. E. (2016). Genome Sequencing and Transposon Mutagenesis of Burkholderia seminalis TC3. 4.2 R3 Identify Genes Contributing to Suppression of Orchid Necrosis Caused by B. gladioli. Molecular Plant-Microbe Interactions, 29(6), 435-446. doi:10.1094/MPMI-02-16-0047-R
Series
Rights Statement
Funding Statement (additional comments about funding)
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2016-06-22T16:08:18Z (GMT). No. of bitstreams: 11 AraujoGenomeSequencingTransposon.pdf: 1434952 bytes, checksum: 2ea5259064806bdf1d4bb42bcfc9d081 (MD5) AraujoGenomeSequencingTransposonFigureS1.pdf: 52184 bytes, checksum: c8d01a9e1c813f45cd19842164bfd09f (MD5) AraujoGenomeSequencingTransposonFigureS2.pdf: 216798 bytes, checksum: f5a89b72d0f81beac6931ed37de8f700 (MD5) AraujoGenomeSequencingTransposonFigureS3.pdf: 256598 bytes, checksum: 40c847f662b2a805159d497096a56cde (MD5) AraujoGenomeSequencingTransposonFigureS4.pdf: 166378 bytes, checksum: 11c3ca663584504139a17e1c32d7d351 (MD5) AraujoGenomeSequencingTransposonFigureS5.pdf: 54289 bytes, checksum: 1a18477a29bc4589ddce27f7cacf12a8 (MD5) AraujoGenomeSequencingTransposonFigureS6.pdf: 118016 bytes, checksum: cc485991a071c734c1598ea87baf84f5 (MD5) AraujoGenomeSequencingTransposonFigureS7.pdf: 211342 bytes, checksum: 0207ad5ab0d8a00d90cf5322f6ba62bb (MD5) AraujoGenomeSequencingTransposonTableS1.pdf: 488725 bytes, checksum: 2f5e0548a07d7cdb490c028474436d45 (MD5) AraujoGenomeSequencingTransposonTableS2.xlsx: 14773 bytes, checksum: 5da01b3f5f9eba73ef88980151800729 (MD5) AraujoGenomeSequencingTransposonTableS3.xlsx: 19969 bytes, checksum: a3b90e811542ba15d0cd169d77d05010 (MD5) Previous issue date: 2016-06
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2016-06-22T16:08:18Z (GMT) No. of bitstreams: 11 AraujoGenomeSequencingTransposon.pdf: 1434952 bytes, checksum: 2ea5259064806bdf1d4bb42bcfc9d081 (MD5) AraujoGenomeSequencingTransposonFigureS1.pdf: 52184 bytes, checksum: c8d01a9e1c813f45cd19842164bfd09f (MD5) AraujoGenomeSequencingTransposonFigureS2.pdf: 216798 bytes, checksum: f5a89b72d0f81beac6931ed37de8f700 (MD5) AraujoGenomeSequencingTransposonFigureS3.pdf: 256598 bytes, checksum: 40c847f662b2a805159d497096a56cde (MD5) AraujoGenomeSequencingTransposonFigureS4.pdf: 166378 bytes, checksum: 11c3ca663584504139a17e1c32d7d351 (MD5) AraujoGenomeSequencingTransposonFigureS5.pdf: 54289 bytes, checksum: 1a18477a29bc4589ddce27f7cacf12a8 (MD5) AraujoGenomeSequencingTransposonFigureS6.pdf: 118016 bytes, checksum: cc485991a071c734c1598ea87baf84f5 (MD5) AraujoGenomeSequencingTransposonFigureS7.pdf: 211342 bytes, checksum: 0207ad5ab0d8a00d90cf5322f6ba62bb (MD5) AraujoGenomeSequencingTransposonTableS1.pdf: 488725 bytes, checksum: 2f5e0548a07d7cdb490c028474436d45 (MD5) AraujoGenomeSequencingTransposonTableS2.xlsx: 14773 bytes, checksum: 5da01b3f5f9eba73ef88980151800729 (MD5) AraujoGenomeSequencingTransposonTableS3.xlsx: 19969 bytes, checksum: a3b90e811542ba15d0cd169d77d05010 (MD5)
  • description.provenance : Submitted by Patricia Black (patricia.black@oregonstate.edu) on 2016-06-22T16:07:56Z No. of bitstreams: 11 AraujoGenomeSequencingTransposon.pdf: 1434952 bytes, checksum: 2ea5259064806bdf1d4bb42bcfc9d081 (MD5) AraujoGenomeSequencingTransposonFigureS1.pdf: 52184 bytes, checksum: c8d01a9e1c813f45cd19842164bfd09f (MD5) AraujoGenomeSequencingTransposonFigureS2.pdf: 216798 bytes, checksum: f5a89b72d0f81beac6931ed37de8f700 (MD5) AraujoGenomeSequencingTransposonFigureS3.pdf: 256598 bytes, checksum: 40c847f662b2a805159d497096a56cde (MD5) AraujoGenomeSequencingTransposonFigureS4.pdf: 166378 bytes, checksum: 11c3ca663584504139a17e1c32d7d351 (MD5) AraujoGenomeSequencingTransposonFigureS5.pdf: 54289 bytes, checksum: 1a18477a29bc4589ddce27f7cacf12a8 (MD5) AraujoGenomeSequencingTransposonFigureS6.pdf: 118016 bytes, checksum: cc485991a071c734c1598ea87baf84f5 (MD5) AraujoGenomeSequencingTransposonFigureS7.pdf: 211342 bytes, checksum: 0207ad5ab0d8a00d90cf5322f6ba62bb (MD5) AraujoGenomeSequencingTransposonTableS1.pdf: 488725 bytes, checksum: 2f5e0548a07d7cdb490c028474436d45 (MD5) AraujoGenomeSequencingTransposonTableS2.xlsx: 14773 bytes, checksum: 5da01b3f5f9eba73ef88980151800729 (MD5) AraujoGenomeSequencingTransposonTableS3.xlsx: 19969 bytes, checksum: a3b90e811542ba15d0cd169d77d05010 (MD5)

Relationships

In Administrative Set:
Last modified: 07/26/2017 Default
Citations:

EndNote | Zotero | Mendeley