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A Fluorescent Antibody Test for Detection of the Rickettsia Causing Disease in Chilean Salmonids Public Deposited

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  • An indirect fluorescent antibody test (IFAT) was developed for detection of the rickettsia that was causing epizootics among salmonids cultured in seawater net-pens in southern Chile. Antiserum against the rickettsial agent was produced in New Zealand white rabbits with a preparation grown in antibiotic-free chinook salmon embryo (CHSE-214) cell cultures and partially purified by a combination of filtration and centrifugation steps. The IFAT was effectively used on blood films, tissue sections, and smears. Two gram-negative and two gram-positive bacterial pathogens of salmonids did not react in this test. Detection of the rickettsial agent has previously been restricted to examination by light microscopy or isolation in salmonid cells. The IFAT provides a simple, rapid, sensitive method for detection of the agent and diagnosis of the disease. The rickettsia is thought to be a member of the tribe Ehrlichieae and was tested by IFAT with sera from animals infected with other rickettsial agents.
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  • Lannan, C. N., Ewing, S. A., & Fryer, J. L. (1991, December). A Fluorescent Antibody Test for Detection of the Rickettsia Causing Disease in Chilean Salmonids. Journal of Aquatic Animal Health, 3(4), 229-234.
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  • This publication is the result, in part, of research sponsored by Oregon Sea Grant with funds from the National Oceanic and Atmospheric Administration Office of Sea Grant, Department of Commerce, under grant NA89AAD- SG108, project R/FSD-14, and by the U.S. Department of Agriculture, Cooperative State Research Service, Special Animal Health and Disease Formula Funds Section 1433.
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  • description.provenance : Approved for entry into archive by Sue Kunda(sue.kunda@oregonstate.edu) on 2011-12-15T00:39:41Z (GMT) No. of bitstreams: 1 AFluorescentAntibodyTest.pdf: 624141 bytes, checksum: 7b87be801bd14968c34d3ff87565d385 (MD5)
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