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https://ir.library.oregonstate.edu/concern/articles/rv042v775

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  • Sporosarcina pasteurii is known to produce calcite or biocement in the presence of urea and Ca²⁺. Herein, we report the use of novel ultramicrosensors such as pH, Ca²⁺, and redox sensors, along with a scanning electrochemical microscope (SECM), to monitor a real-time, bacteria-mediated urea hydrolysis process and subsequent changes in morphology due to CaCO₃ precipitation. We report that the surface pH of a live biofilm changed rapidly from 7.4 to 9.2 within 2 min, whereas similar fast depletion (10 min) of Ca²⁺ was observed from 85 mM to 10 mM in the presence of a high urea (10 g L⁻¹) brine solution at 23 °C. Both the pH and the Ca²⁺ concentration profiles were extended up to 600 μm from the biofilm surface, whereas the bulk chemical composition of the brine solution remained constant over the entire 4 h of SECM experiments. In addition, we observed a change in biofilm surface morphology and an increase in overall biofilm height of 50 μm after 4 h of precipitation. Electron microscopy confirmed the changes in surface morphology and formation of CaCO₃ crystals. Development of the Ca²⁺ profile took 10 min, whereas that of the pH profile took 2 min. This finding indicates that the initial urea hydrolysis process is fast and limited by urease or number of bacteria, whereas later CaCO₃ formation and growth of crystals is a slow chemical process. The ultramicrosensors and approaches employed here are capable of accurately characterizing bioremediation on temporal and spatial scales pertinent to the microbial communities and the processes they mediate.
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  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2016-06-23T15:35:05Z (GMT) No. of bitstreams: 2 HarrisRealTimeMonitoring.pdf: 3726929 bytes, checksum: 94666857e9c0d72f5c885f54465cf80e (MD5) HarrisRealTimeMonitoringSupportingInformation.pdf: 397735 bytes, checksum: 8747b496114492bd765cdda7eeac4291 (MD5)
  • description.provenance : Submitted by Patricia Black (patricia.black@oregonstate.edu) on 2016-06-23T15:32:57Z No. of bitstreams: 2 HarrisRealTimeMonitoring.pdf: 3726929 bytes, checksum: 94666857e9c0d72f5c885f54465cf80e (MD5) HarrisRealTimeMonitoringSupportingInformation.pdf: 397735 bytes, checksum: 8747b496114492bd765cdda7eeac4291 (MD5)
  • description.provenance : Made available in DSpace on 2016-06-23T15:35:05Z (GMT). No. of bitstreams: 2 HarrisRealTimeMonitoring.pdf: 3726929 bytes, checksum: 94666857e9c0d72f5c885f54465cf80e (MD5) HarrisRealTimeMonitoringSupportingInformation.pdf: 397735 bytes, checksum: 8747b496114492bd765cdda7eeac4291 (MD5) Previous issue date: 2016-05-21

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