Species-Specific PCR Assays for Differentiating Heterodera filipjevi and H. avenae Public Deposited

http://ir.library.oregonstate.edu/concern/articles/s7526f12k

To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The published article is copyrighted by the American Phytopathological Society and can be found at:  http://apsjournals.apsnet.org/loi/pdis.

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  • Heterodera avenae and H. filipjevi are economically important cyst nematodes that restrict production of cereal crops in the Pacific Northwest United States and elsewhere in the world. Identification of these two species is critical for recommending and implementing effective management practices. Primers were designed from the internal transcribed spacer (ITS) regions of H. avenae and H. filipjevi ribosomal DNA. The primers were highly specific when examined on target isolates but did not amplify DNA from nontarget Heterodera, Globodera, Meloidogyne, Pratylenchus, and other nematode species tested. Polymerase chain reaction (PCR) and amplification conditions were established, and H. avenae and H. filipjevi were clearly distinguished by PCR fragments of 242 and 170 bp, respectively. Robust PCR amplification was achieved with DNA extracted from a single egg or second-stage juvenile (J2) using a laboratory-made worm lysis buffer, and DNA from 0.5 egg or J2 using a commercial kit. The PCR assays were successfully employed for differentiation of H. filipjevi and H. avenae populations collected from eight locations in three Pacific Northwest states. This is the first report of a species-specific ITS PCR assay to detect and identify H. filipjevi. The assays for both species will enhance diagnosis of cereal cyst nematode species in infested fields.
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  • Yan, G., Smiley, R. W., Okubara, P. A., & Skantar, A. M. (2013). Species-Specific PCR Assays for Differentiating Heterodera filipjevi and H. avenae. Plant Disease, 97(12), 1611-1619. doi:10.1094/PDIS-01-13-0064-RE
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  • description.provenance : Made available in DSpace on 2014-03-18T16:21:39Z (GMT). No. of bitstreams: 1 YanGuipingCropSoilScienceSpecies-SpecificPCR.pdf: 358570 bytes, checksum: a356386df2ee398fb538b07fc79c1a4f (MD5) Previous issue date: 2013-12
  • description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2014-03-18T16:21:39Z (GMT) No. of bitstreams: 1 YanGuipingCropSoilScienceSpecies-SpecificPCR.pdf: 358570 bytes, checksum: a356386df2ee398fb538b07fc79c1a4f (MD5)
  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2014-03-18T16:21:03Z No. of bitstreams: 1 YanGuipingCropSoilScienceSpecies-SpecificPCR.pdf: 358570 bytes, checksum: a356386df2ee398fb538b07fc79c1a4f (MD5)

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