The Type II Secretion Pathway in Vibrio cholerae Is Characterized by Growth Phase-Dependent Expression of Exoprotein Genes and Is Positively Regulated by σ[superscript E] Public Deposited

http://ir.library.oregonstate.edu/concern/articles/sb397998g

This is the publisher’s final pdf. The published article is copyrighted by the American Society for Microbiology and can be found at:  http://iai.asm.org/.

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Vibrio cholerae, an etiological agent of cholera, circulates between aquatic reservoirs and the human gastrointestinal tract. The type II secretion (T2S) system plays a pivotal role in both stages of the lifestyle by exporting multiple proteins, including cholera toxin. Here, we studied the kinetics of expression of genes encoding the T2S system and its cargo proteins. We have found that under laboratory growth conditions, the T2S complex was continuously expressed throughout V. cholerae growth, whereas there was growth phase-dependent transcriptional activity of genes encoding different cargo proteins. Moreover, exposure of V. cholerae to different environmental cues encountered by the bacterium in its life cycle induced transcriptional expression of T2S. Subsequent screening of a V. cholerae genomic library suggested that σ[superscript E] stress response, phosphate metabolism, and the second messenger 3',5'-cyclic diguanylic acid (c-di-GMP) are involved in regulating transcriptional expression of T2S. Focusing on σ[superscript E], we discovered that the upstream region of the T2S operon possesses both the consensus σ[superscript E] and σ⁷⁰ signatures, and deletion of the σ[superscript E] binding sequence prevented transcriptional activation of T2S by RpoE. Ectopic overexpression of σ[superscript E] stimulated transcription of T2S in wild-type and isogenic ΔrpoE strains of V. cholerae, providing additional support for the idea that the T2S complex belongs to the σ[superscript E] regulon. Together, our results suggest that the T2S pathway is characterized by the growth phase-dependent expression of genes encoding cargo proteins and requires a multifactorial regulatory network to ensure appropriate kinetics of the secretory traffic and the fitness of V. cholerae in different ecological niches.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Zielke, R. A., Simmons, R. S., Park, B. R., Nonogaki, M., & Emerson, S. (2014). The Type II Secretion Pathway in Vibrio cholerae Is Characterized by Growth Phase-Dependent Expression of Exoprotein Genes and Is Positively Regulated by σ[superscript E]. Infection and Immunity, 82(7), 2788-2801. doi:10.1128/IAI.01292-13
Series
Rights Statement
Funding Statement (additional comments about funding)
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2014-08-11T19:24:06Z (GMT) No. of bitstreams: 2 ZielkeRyszardPharmacyTypeIISecretion.pdf: 1407493 bytes, checksum: 21944819ee28d6f2648f0395201d0275 (MD5) ZielkeRyszardPharmacyTypeIISecretion_SupplementalMaterial.pdf: 5330397 bytes, checksum: 07863be53d9ba2bd79d90bfb0ee856af (MD5)
  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2014-08-11T19:23:53Z No. of bitstreams: 2 ZielkeRyszardPharmacyTypeIISecretion.pdf: 1407493 bytes, checksum: 21944819ee28d6f2648f0395201d0275 (MD5) ZielkeRyszardPharmacyTypeIISecretion_SupplementalMaterial.pdf: 5330397 bytes, checksum: 07863be53d9ba2bd79d90bfb0ee856af (MD5)
  • description.provenance : Made available in DSpace on 2014-08-11T19:24:06Z (GMT). No. of bitstreams: 2 ZielkeRyszardPharmacyTypeIISecretion.pdf: 1407493 bytes, checksum: 21944819ee28d6f2648f0395201d0275 (MD5) ZielkeRyszardPharmacyTypeIISecretion_SupplementalMaterial.pdf: 5330397 bytes, checksum: 07863be53d9ba2bd79d90bfb0ee856af (MD5) Previous issue date: 2014-07

Relationships

In Administrative Set:
Last modified: 07/26/2017 Default
Citations:

EndNote | Zotero | Mendeley

Items