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Full membrane spanning self-assembled monolayers as model systems for UHV-based studies of cell-penetrating peptides Public Deposited

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  • Biophysical studies of the interaction of peptides with model membranes provide a simple yet effective approach to understand the transport of peptides and peptide based drug carriers across the cell membrane. Herein, the authors discuss the use of self-assembled monolayers fabricated from the full membrane-spanning thiol (FMST) 3-((14-((40-((5-methyl-1-phenyl-35-(phytanyl)oxy- 6,9,12,15,18,21,24,27,30,33,37-undecaoxa-2,3-dithiahenpentacontan-51-yl)oxy)-[1,10-biphenyl]-4- yl)oxy)tetradecyl)oxy)-2-(phytanyl)oxy glycerol for ultrahigh vacuum (UHV) based experiments. UHV-based methods such as electron spectroscopy and mass spectrometry can provide important information about how peptides bind and interact with membranes, especially with the hydrophobic core of a lipid bilayer. Near-edge x-ray absorption fine structure spectra and x-ray photoelectron spectroscopy (XPS) data showed that FMST forms UHV-stable and ordered films on gold. XPS and time of flight secondary ion mass spectrometry depth profiles indicated that a proline-rich amphipathic cell-penetrating peptide, known as sweet arrow peptide is located at the outer perimeter of the model membrane.
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  • Franz, J., Graham, D. J., Schmüser, L., Baio, J. E., Lelle, M., Peneva, K., ... & Weidner, T. (2015). Full membrane spanning self-assembled monolayers as model systems for UHV-based studies of cell-penetrating peptides. Biointerphases, 10(1), 019009. doi:10.1116/1.4908164
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  • 10
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  • J.F. and T.W. thank Hao Lu for help with the XPS thicknessdetermination and the Deutsche Forschungsgemeinschaft (WE4478/2-1) and European Union Marie Curie Program forsupport of this work (CIG grant #322124). This work is part ofthe research program of the Max Planck Society. J.F.gratefully acknowledges support by the International MaxPlanck Research School. D.J.G. and D.G.C. thank grant EB-002027 from the U.S. National Institutes of Health for supportof the ToF-SIMS experiments. NEXAFS spectra werecollected at the National Synchrotron Light Source (NSLS),Brookhaven National Laboratory, which was supported by theU.S. Department of Energy, Division of Materials Science andDivision of Chemical Sciences.
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  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2015-05-05T17:30:16ZNo. of bitstreams: 1BaioJoeChemBioEnvEngFullMembraneSpanning.pdf: 936304 bytes, checksum: 9af6932cb0f02f2f9c6e07f9364a62d2 (MD5)
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