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MinotEthanPhysicsGrapheneBiotransistorInterfacedWithNitrifyingBiofilm(SupportingInformation).pdf Public Deposited

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https://ir.library.oregonstate.edu/concern/articles/t435gj765

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  • Using a graphene field-effect transistor biosensor, we monitored the pH inside a living biofilm with fast temporal resolution (~ 1 s) over multi-hour time periods. The atomically-thin sensor is positioned between the biofilm and a supporting silicon oxide surface, providing non-invasive access to conditions at the base of the biofilm. We determine the transient changes in pH when the biofilm metabolizes substrate molecules and when it is exposed to biocide. The pH resolution is approximately 0.01 pH units when using 1-second time averaging. The sensor drift is approximately 0.01 pH units per hour. Our results demonstrate the potential of this technology to study biofilm metabolism and monitor biofilm health.
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