Genome-wide mapping of chromatin state of mouse forelimbs Public Deposited

http://ir.library.oregonstate.edu/concern/articles/tx31qk37x

This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by Dove Medical Press Ltd. The published article can be found at:  http://www.dovepress.com/open-access-bioinformatics-journal.

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • BACKGROUND: Cell types are defined at the molecular level during embryogenesis by a process called pattern formation and created by the selective utilization of combinations of sequence-specific transcription factors. Developmental programs define the sets of genes that are available to each particular cell type, and real-time biochemical signaling interactions define the extent to which these sets are used at any given time and place. Gene expression is regulated through the integrated action of many cis-regulatory elements, including core promoters, enhancers, silencers, and insulators. The chromatin state in developing body parts provides a code to cellular populations that directs their cell fates. Chromatin profiling has been a method of choice for mapping regulatory sequences in cells that go through developmental transitions. RESULTS: We used antibodies against histone H3 lysine 4 trimethylations, a modification associated with promoters and open/active chromatin, histone H3 lysine 27 trimethylations associated with Polycomb-repressed regions, and ribonucleic acid polymerase II associated with transcriptional initiation to identify the chromatin state signature of the mouse forelimb during mid-gestation at embryonic day 12. The families of genes marked included those related to transcriptional regulation and embryogenesis. One-third of the marked genes were transcriptionally active, whereas only a small fraction were bivalent marked. Sequence-specific transcription factors that were activated were involved in cell specification, including bone and muscle formation. CONCLUSION: Our results demonstrate that embryonic limb cells do not exhibit the plasticity of the embryonic stem cells but rather are programmed for a finer tuning for cell lineage specification.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Eng, D., Vogel, W. K., Flann, N. S., Gross, M. K. & Kioussi, C. (2014). Genome-wide mapping of chromatin state of mouse forelimbs. Open Access Bioinformatics, 6, 1-11. doi:10.2147/OAB.S59043
Series
Keyword
Rights Statement
Funding Statement (additional comments about funding)
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2014-10-15T15:01:08Z (GMT) No. of bitstreams: 2 license_rdf: 1379 bytes, checksum: da3654ba11642cda39be2b66af335aae (MD5) EngDianaPharmacyGenome-WideMapping.pdf: 2997946 bytes, checksum: ff6afc9239a5c76197111b6cce4d586c (MD5)
  • description.provenance : Made available in DSpace on 2014-10-15T15:01:08Z (GMT). No. of bitstreams: 2 license_rdf: 1379 bytes, checksum: da3654ba11642cda39be2b66af335aae (MD5) EngDianaPharmacyGenome-WideMapping.pdf: 2997946 bytes, checksum: ff6afc9239a5c76197111b6cce4d586c (MD5) Previous issue date: 2014-09-10
  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2014-10-15T15:00:42Z No. of bitstreams: 2 license_rdf: 1379 bytes, checksum: da3654ba11642cda39be2b66af335aae (MD5) EngDianaPharmacyGenome-WideMapping.pdf: 2997946 bytes, checksum: ff6afc9239a5c76197111b6cce4d586c (MD5)

Relationships

In Administrative Set:
Last modified: 07/26/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items