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Toward autonomous measurements of photosynthetic electron transport rates: An evaluation of active fluorescence-based measurements of photochemistry

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https://ir.library.oregonstate.edu/concern/articles/v405sc23c

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  • This study presents a methods evaluation and intercalibration of active fluorescence-based measurements of the quantum yield (ϕ'ₚₛᵢᵢ) and absorption coefficient (aₚₛᵢᵢ) of photosystem II (PSII) photochemistry. Measurements of ϕ'ₚₛᵢᵢ, aₚₛᵢᵢ, and irradiance (E) can be scaled to derive photosynthetic electron transport rates (Pₑ), the process that fuels phytoplankton carbon fixation and growth. Bio-optical estimates of ϕ'ₚₛᵢᵢ and aₚₛᵢᵢ were evaluated using 10 phytoplankton cultures across different pigment groups with varying bio-optical absorption characteristics on six different fast-repetition rate fluorometers that span two different manufacturers and four different models. Culture measurements of ϕ'ₚₛᵢᵢ and the effective absorption cross section of PSII photochemistry (σₚₛᵢᵢ, a constituent of aₚₛᵢᵢ) showed a high degree of correspondence across instruments, although some instrument-specific biases are identified. A range of approaches have been used in the literature to estimate aₚₛᵢᵢ(λ) and are evaluated here.With the exception of ex situ aₚₛᵢᵢ(λ) estimates from paired σₚₛᵢᵢ and PSII reaction center concentration ([RCII]) measurements, the accuracy and precision of in situ aₚₛᵢᵢ(λ) methodologies are largely determined by the variance of method-specific coefficients. The accuracy and precision of these coefficients are evaluated, compared to literature data, and discussed within a framework of autonomous Pₑ measurements. This study supports the application of an instrument-specific calibration coefficient (KR) that scales minimum fluorescence in the dark (F₀) to aₚₛᵢᵢ as both the most accurate in situ measurement of aₚₛᵢᵢ, and the methodology best suited for highly resolved autonomous Pₑ measurements.
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  • Silsbe, G. M., Oxborough, K., Suggett, D. J., Forster, R. M., Ihnken, S., Komárek, O., ... & Kromkamp, J. C. (2015). Toward autonomous measurements of photosynthetic electron transport rates: An evaluation of active fluorescence‐based measurements of photochemistry. Limnology and Oceanography: Methods, 13(3), 138-155. doi:10.1002/lom3.10014
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  • 13
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  • 3
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  • This is the publisher’s final pdf. The article is copyrighted by the Association for the Sciences of Limnology and Oceanography and published by John Wiley & Sons Ltd.
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