- Background: Potato virus Y (PVY) is one of the most important plant viruses affecting potato production. The
interactions between potato and PVY are complex and the outcome of the interactions depends on the potato
genotype, the PVY strain, and the environmental conditions. A potato cultivar can induce resistance to a specific
PVY strain, yet be susceptible to another. How a single potato cultivar responds to PVY in both compatible and
incompatible interactions is not clear.
Results: In this study, we used RNA-sequencing (RNA-Seq) to investigate and compare the transcriptional changes
in leaves of potato upon inoculation with PVY. We used two potato varieties: Premier Russet, which is resistant to
the PVY strain O (PVYᴼ) but susceptible to the strain NTN (PVYᴺᵀᴺ), and Russet Burbank, which is susceptible to all
PVY strains that have been tested. Leaves were inoculated with PVYᴼ or PVYᴺᵀᴺ, and samples were collected 4 and
10 h post inoculation (hpi). A larger number of differentially expressed (DE) genes were found in the compatible
reactions compared to the incompatible reaction. For all treatments, the majority of DE genes were down-regulated
at 4 hpi and up-regulated at 10 hpi. Gene Ontology enrichment analysis showed enrichment of the biological
process GO term “Photosynthesis, light harvesting” specifically in PVYᴼ-inoculated Premier Russet leaves, while
the GO term “nucleosome assembly” was largely overrepresented in PVYᴺᵀᴺ-inoculated Premier Russet leaves and
PVYᴼ-inoculated Russet Burbank leaves but not in PVYᴼ-inoculated Premier Russet leaves. Fewer genes were DE
over 4-fold in the incompatible reaction compared to the compatible reactions. Amongst these, five genes were
DE only in PVYᴼ-inoculated Premier Russet leaves, and all five were down-regulated. These genes are predicted to
encode for a putative ABC transporter, a MYC2 transcription factor, a VQ-motif containing protein, a non-specific
lipid-transfer protein, and a xyloglucan endotransglucosylase-hydroxylase.
Conclusions: Our results show that the incompatible and compatible reactions in Premier Russet shared more
similarities, in particular during the initial response, than the compatible reactions in the two different hosts. Our
results identify potential key processes and genes that determine the fate of the reaction, compatible or
incompatible, between PVY and its host.
- Goyer, A., Hamlin, L., Crosslin, J. M., Buchanan, A., & Chang, J. H. (2015). RNA-Seq analysis of resistant and susceptible potato varieties during the early stages of potato virus Y infection. BMC genomics, 16:472. doi:10.1186/s12864-015-1666-2
|Funding Statement (additional comments about funding)
- This project was supported by funding from the Oregon and Washington state potato commissions. Work in the laboratory of JHC is supported by funding from the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R01GM104977. Publication of this article was funded by the Oregon State University Libraries & Press Open Access Fund.
- Additional files can be located at: http://www.biomedcentral.com/1471-2164/16/472/additional
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