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High aspect ratio nanoimprinted grooves of poly(lactic-co-glycolic acid) control the length and direction of retraction fibers during fibroblast cell division

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https://ir.library.oregonstate.edu/concern/articles/vq27zq38k

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  • Retraction fibers (RFs) determine orientation of the cell division axis and guide the spreading of daughter cells. Long and unidirectional RFs, which are especially apparent during mitosis of cells in three-dimensional (3D) environments, enable improved control over cell fate, following division. However, 3D gel environments lack the cues necessary for predetermining the orientation of RFs to direct tissue architecture. While patterning of focal adhesion regions by microcontact printing can determine orientation of the RFs through enhancing focal adhesion numbers along particular directions, the RFs remain short due to the two-dimensional culture environment. Herein, the authors demonstrate that nanoimprinted grooves of polylactic acid glycolic acid (PLGA) with a high aspect ratio (A.R. of 2.0) can provide the cues necessary to control the direction of RFs, as well as enable the maintenance of long and unidirectional RFs as observed within 3D cultures, while the same is not possible with PLGA grooves of lower A.R. (1.0 or lower). Based on enhanced levels of contact guidance of premitotic fibroblast protrusions at high A.R. grooves and deeper levels of focal adhesion due to filopodia extensions into these grooves, it is suggested that submicron (800 nm width) PLGA grooves with A.R. of 2 are capable of supporting mechanical forces from cell protrusions to a greater depth, thereby enabling the maintenance of the protrusions as long and unidirectional RFs during cell division. Given the scalability and versatility of nanoimprint techniques, the authors envision a platform for designing nanostructures to direct tissue regeneration and developmental biology.
  • This is the publisher’s final pdf. The article is copyrighted by the American Vacuum Society and published by Springer. It can be found at: http://link.springer.com/journal/13758
  • See supplementary material at http://dx.doi.org/10.1116/1.4936589 for movies on cell trajectories during division on the grooves, as well as for alignment and elongation of pre-mitotic protrusions on PLGA grooves.
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  • Su, Y. H., Chiang, P. C., Cheng, L. J., Lee, C. H., Swami, N. S., & Chou, C. F. (2015). High aspect ratio nanoimprinted grooves of poly (lactic-co-glycolic acid) control the length and direction of retraction fibers during fibroblast cell division. Biointerphases, 10(4), 041008. doi:10.1116/1.4936589
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  • 10
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  • 4
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  • This work was supported by the Academia Sinica Research Program on Nanoscience and Nanotechnology, Academia Sinica Integrated Thematic Project (AS-103-TP-A01), the Minister of Science and Technology, Taiwan (102-2112-M-001-005-MY3 and 103-2923-M-001-007-MY3), Asian Office for Aerospace Research and Development (No. FA2386-12-1-4002), and USA NSF Award No. 1332329.
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file details ChengLiJingEECSHighAspectRatio.pdf 2017-07-28 Público Baixar