Unraveling Ultrafast Photoinduced Proton Transfer Dynamics in a Fluorescent Protein Biosensor for Ca²⁺ Imaging Public Deposited

http://ir.library.oregonstate.edu/concern/articles/w66345177

This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by John Wiley & Sons, Inc., and can be found at:  http://onlinelibrary.wiley.com/journal/10.1002/%28ISSN%291521-3765

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  • Unraveling Ultrafast Photoinduced Proton Transfer Dynamics in a Fluorescent Protein Biosensor for Ca2+ Imaging
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  • Imaging Ca²⁺ dynamics in living systems holds great potential to advance neuroscience and cellular biology. G-GECO1.1 is an intensiometric fluorescent protein Ca²⁺-biosensor with a Thr-Tyr-Gly chromophore. The protonated chromophore emits green upon photoexcitation via excited-state proton transfer (ESPT). Upon Ca²⁺ binding, a significant population of the chromophores becomes deprotonated. It remains elusive how the chromophore structurally evolves prior to and during ESPT, and how it is affected by Ca²⁺. We use femtosecond stimulated Raman spectroscopy to dissect ESPT in both the Ca²⁺-free and bound states. The protein chromophores exhibit a sub-200 fs vibrational frequency shift due to coherent small-scale proton motions. After wavepackets move out of the Franck-Condon region, ESPT gets faster in the Ca²⁺-bound protein, indicative of the formation of a more hydrophilic environment. These results reveal the governing structure-function relationship of Ca²⁺-sensing protein biosensors.
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  • Tang, L., Liu, W., Wang, Y., Zhao, Y., Oscar, B. G., Campbell, R. E., & Fang, C. (2015). Unraveling ultrafast photoinduced proton transfer dynamics in a fluorescent protein biosensor for Ca²⁺ imaging. Chemistry-A European Journal, 21(17), 6481-6490. doi:10.1002/chem.201500491
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  • description.provenance : Approved for entry into archive by Deanne Bruner(deanne.bruner@oregonstate.edu) on 2015-10-23T18:27:44Z (GMT) No. of bitstreams: 1 FangChongChemistryUnraveling UltrafastPhotoinducedProtonTransferDynamics.pdf: 2852525 bytes, checksum: 3efe31ffafdb2cec59662699f18511c3 (MD5)
  • description.provenance : Submitted by Deanne Bruner (deanne.bruner@oregonstate.edu) on 2015-10-23T18:26:11Z No. of bitstreams: 1 FangChongChemistryUnraveling UltrafastPhotoinducedProtonTransferDynamics.pdf: 2852525 bytes, checksum: 3efe31ffafdb2cec59662699f18511c3 (MD5)
  • description.provenance : Made available in DSpace on 2015-10-23T18:27:44Z (GMT). No. of bitstreams: 1 FangChongChemistryUnraveling UltrafastPhotoinducedProtonTransferDynamics.pdf: 2852525 bytes, checksum: 3efe31ffafdb2cec59662699f18511c3 (MD5) Previous issue date: 2015-04-20

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