Article

 

Variable Suites of Non-effector Genes Are Co-regulated in the Type III Secretion Virulence Regulon across the Pseudomonas syringae Phylogeny Public Deposited

Analytics
https://ir.library.oregonstate.edu/concern/articles/wd375x80c

This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by the Public Library of Science. The published article can be found at:  http://www.plospathogens.org/.

Descriptions

Attribute NameValues
Creator
Abstract
  • Pseudomonas syringae is a phylogenetically diverse species of Gram-negative bacterial plant pathogens responsible for crop diseases around the world. The HrpL sigma factor drives expression of the major P. syringae virulence regulon. HrpL controls expression of the genes encoding the structural and functional components of the type III secretion system (T3SS) and the type three secreted effector proteins (T3E) that are collectively essential for virulence. HrpL also regulates expression of an under-explored suite of non-type III effector genes (non-T3E), including toxin production systems and operons not previously associated with virulence. We implemented and refined genome-wide transcriptional analysis methods using cDNA-derived high-throughput sequencing (RNA-seq) data to characterize the HrpL regulon from six isolates of P. syringae spanning the diversity of the species. Our transcriptomes, mapped onto both complete and draft genomes, significantly extend earlier studies. We confirmed HrpL-regulation for a majority of previously defined T3E genes in these six strains. We identified two new T3E families from P. syringae pv. oryzae 1_6, a strain within the relatively underexplored phylogenetic Multi-Locus Sequence Typing (MLST) group IV. The HrpL regulons varied among strains in gene number and content across both their T3E and non-T3E gene suites. Strains within MLST group II consistently express the lowest number of HrpL-regulated genes. We identified events leading to recruitment into, and loss from, the HrpL regulon. These included gene gain and loss, and loss of HrpL regulation caused by group-specific cis element mutations in otherwise conserved genes. Novel non-T3E HrpL-regulated genes include an operon that we show is required for full virulence of P. syringae pv. phaseolicola 1448A on French bean. We highlight the power of integrating genomic, transcriptomic, and phylogenetic information to drive concise functional experimentation and to derive better insight into the evolution of virulence across an evolutionarily diverse pathogen species.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Mucyn TS, Yourstone S, Lind AL, Biswas S, Nishimura MT, et al. (2014) Variable Suites of Non-effector Genes Are Co-regulated in the Type III Secretion Virulence Regulon across the Pseudomonas syringae Phylogeny. PLoS Pathogens, 10(1): e1003807. doi:10.1371/journal.ppat.1003807
Journal Title
Journal Volume
  • 10
Journal Issue/Number
  • 1
Rights Statement
Funding Statement (additional comments about funding)
  • This work was supported by NIH training grant Predoctoral Training Program in Bioinformatics and Computational Biology T32 GM067553-06 (SY, CDJ, JLD) and R01-GM066025 (CDJ, JLD, SRG). JSC was supported by the National Research Initiative Competitive Grants Program Grant no. 2008-35600-04691 (JHC). JLD is an HHMI Investigator and this work was funded in part by the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation (via Grant GBMF3030).
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2014-05-07T20:29:08Z No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) CumbieJasonBotanyPlantPathologyVariableSuitesNon-Effector.pdf: 2438451 bytes, checksum: 50b49efbe60c392398a6b263f43c5034 (MD5) CumbieJasonBotanyPlantPathologyVariableSuitesNon-Effector_SupportingInformation.zip: 6374484 bytes, checksum: ba5f959f9a02016d63e961db8c0e6e9f (MD5)
  • description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2014-05-07T20:29:31Z (GMT) No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) CumbieJasonBotanyPlantPathologyVariableSuitesNon-Effector.pdf: 2438451 bytes, checksum: 50b49efbe60c392398a6b263f43c5034 (MD5) CumbieJasonBotanyPlantPathologyVariableSuitesNon-Effector_SupportingInformation.zip: 6374484 bytes, checksum: ba5f959f9a02016d63e961db8c0e6e9f (MD5)
  • description.provenance : Made available in DSpace on 2014-05-07T20:29:31Z (GMT). No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) CumbieJasonBotanyPlantPathologyVariableSuitesNon-Effector.pdf: 2438451 bytes, checksum: 50b49efbe60c392398a6b263f43c5034 (MD5) CumbieJasonBotanyPlantPathologyVariableSuitesNon-Effector_SupportingInformation.zip: 6374484 bytes, checksum: ba5f959f9a02016d63e961db8c0e6e9f (MD5) Previous issue date: 2014-01-02

Relationships

Parents:

This work has no parents.

Items

Thumbnail Title Date Uploaded Visibility Actions
CumbieJasonBotanyPlantPathologyVariableSuitesNon-Effector_SupportingInformation.zip 07/25/2017 Public
CumbieJasonBotanyPlantPathologyVariableSuitesNon-Effector.pdf 07/25/2017 Public