Interactions of yeast dynein with dynein light chain and dynactin: General implications for intrinsically disordered duplex scaffolds in multi-protein assemblies Public Deposited

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  • Intrinsically disordered protein (IDP) duplexes composed of two IDP chains cross-linked by bivalent partner proteins form scaffolds for assembly of multiprotein complexes. The N-terminal domain of dynein intermediate chain (N-IC) is one such IDP that forms a bivalent scaffold with multiple dynein light chains including LC8, a hub protein that promotes duplex formation of diverse IDP partners. N-IC also binds a subunit of the dynein regulator, dynactin. Here we characterize interactions of a yeast ortholog of N-IC (N-Pac11) with yeast LC8 (Dyn2) or with the intermediate chain-binding subunit of yeast dynactin (Nip100). Residue level changes in Pac11 structure are monitored by NMR spectroscopy, and binding energetics are monitored by isothermal titration calorimetry (ITC). N-Pac11 is monomeric and primarily disordered except for a single α-helix (SAH) at the N terminus and a short nascent helix, LH, flanked by the two Dyn2 recognition motifs. Upon binding Dyn2, the only Pac11 residues making direct protein-protein interactions are in and immediately flanking the recognition motifs. Dyn2 binding also orders LH residues of Pac11. Upon binding Nip100, only Pac11 SAH residues make direct protein-protein interactions, but LH residues at a distant sequence position and L1 residues in an adjacent linker are also ordered. The long distance, ligand-dependent ordering of residues reveals new elements of dynamic structure within IDP linker regions.
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  • Jie, J., Löhr, F., & Barbar, E. (2015). Interactions of Yeast Dynein with Dynein Light Chain and Dynactin: General implications for intrinsically disordered duplex scaffolds in multi-protein assemblies. Journal of Biological Chemistry, 290(39), 23863-23874. doi:10.1074/jbc.M115.649715
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  • 290
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  • 39
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  • This work is supported by National Institutes of Health Grant GM 084276 to EB. We acknowledge thesupport of the protein core facility in the OSU Environmental Health Sciences Center (NIH/NIEHS00210), and access to the Research Infrastructure activity in the 7th Framework Programme of the EC(Project number: 261863, Bio-NMR) (Frankfurt, Germany).
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