Delivering the cell-impermeable DNA 'light-switching' Ru(II) complexes preferentially into live-cell nucleus via an unprecedented ion-pairing method Public Deposited

http://ir.library.oregonstate.edu/concern/articles/ww72bg64v

Electronic supplementary information (ESI) available. CCDC 1046781. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c5sc03796d

This is the publisher’s final pdf. The published article is copyrighted by the Royal Society of Chemistry and can be found at:  http://pubs.rsc.org/en/Journals/JournalIssues/SC#!recentarticles&adv

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • The dipyridophenazine (dppz) based ruthenium polypyridyl complexes are known as molecular ‘light-switches’ for DNA. This property is poised to serve in diagnostic and therapeutic applications, but the poor cellular uptake restricts their use in live cells. Herein, we show that the cellular uptake, and more interestingly and surprisingly, the nuclear uptake of cell-impermeable Ru(II)–polypyridyl cationic complexes such as [Ru(bpy)₂(dppz)]²⁺ were remarkably enhanced by three structurally unrelated biochemical agents (pentachlorophenol, carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone and tolfenamic acid), by forming lipophilic and relatively stable ion-pair complexes, via a passive diffusion mechanism. Enantioselective imaging of live-cell nuclear DNA was observed between the two chiral forms of Ru(II) complexes. This represents the first report of an unprecedented new method for delivering the DNA ‘light-switching’ Ru(II) complexes into the nucleus of living cells via ion-pairing, which could serve as a promising general live-cell delivery method for other potentially bio-medically important but cell-impermeable metal complexes.
License
Resource Type
DOI
Date Available
Date Issued
Citation
  • Zhu, B. Z., Chao, X. J., Huang, C. H., & Li, Y. (2016). Delivering the cell-impermeable DNA ‘light-switching’Ru (ii) complexes preferentially into live-cell nucleus via an unprecedented ion-pairing method. Chemical Science, 7(7), 4016-4023. doi:10.1039/c5sc03796d
Series
Rights Statement
Funding Statement (additional comments about funding)
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2016-07-29T17:31:11Z (GMT). No. of bitstreams: 4 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) ZhuDeliveringtheCellImpermeable.pdf: 1750198 bytes, checksum: a1fbb460ccbf7a4e04dce42f7eecd08f (MD5) ZhuDeliveringtheCellImpermeableSuppInfo.pdf: 1400185 bytes, checksum: a08cee3a8c6900262076fdb32b4562d2 (MD5) ZhuDeliveringtheCellImpermeableCellStructureData.cif: 31850 bytes, checksum: 2b6a44dacd51a05ab66782ea86acd08e (MD5) Previous issue date: 2016
  • description.provenance : Submitted by Patricia Black (patricia.black@oregonstate.edu) on 2016-07-29T17:30:43Z No. of bitstreams: 4 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) ZhuDeliveringtheCellImpermeable.pdf: 1750198 bytes, checksum: a1fbb460ccbf7a4e04dce42f7eecd08f (MD5) ZhuDeliveringtheCellImpermeableSuppInfo.pdf: 1400185 bytes, checksum: a08cee3a8c6900262076fdb32b4562d2 (MD5) ZhuDeliveringtheCellImpermeableCellStructureData.cif: 31850 bytes, checksum: 2b6a44dacd51a05ab66782ea86acd08e (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2016-07-29T17:31:11Z (GMT) No. of bitstreams: 4 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) ZhuDeliveringtheCellImpermeable.pdf: 1750198 bytes, checksum: a1fbb460ccbf7a4e04dce42f7eecd08f (MD5) ZhuDeliveringtheCellImpermeableSuppInfo.pdf: 1400185 bytes, checksum: a08cee3a8c6900262076fdb32b4562d2 (MD5) ZhuDeliveringtheCellImpermeableCellStructureData.cif: 31850 bytes, checksum: 2b6a44dacd51a05ab66782ea86acd08e (MD5)

Relationships

In Administrative Set:
Last modified: 10/16/2017 Default
Citations:

EndNote | Zotero | Mendeley

Items