Article
 

Delivering the cell-impermeable DNA 'light-switching' Ru(II) complexes preferentially into live-cell nucleus via an unprecedented ion-pairing method

Pubblico Deposited

Contenuto scaricabile

Scarica il pdf
https://ir.library.oregonstate.edu/concern/articles/ww72bg64v

Descriptions

Attribute NameValues
Creator
Abstract
  • The dipyridophenazine (dppz) based ruthenium polypyridyl complexes are known as molecular ‘light-switches’ for DNA. This property is poised to serve in diagnostic and therapeutic applications, but the poor cellular uptake restricts their use in live cells. Herein, we show that the cellular uptake, and more interestingly and surprisingly, the nuclear uptake of cell-impermeable Ru(II)–polypyridyl cationic complexes such as [Ru(bpy)₂(dppz)]²⁺ were remarkably enhanced by three structurally unrelated biochemical agents (pentachlorophenol, carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone and tolfenamic acid), by forming lipophilic and relatively stable ion-pair complexes, via a passive diffusion mechanism. Enantioselective imaging of live-cell nuclear DNA was observed between the two chiral forms of Ru(II) complexes. This represents the first report of an unprecedented new method for delivering the DNA ‘light-switching’ Ru(II) complexes into the nucleus of living cells via ion-pairing, which could serve as a promising general live-cell delivery method for other potentially bio-medically important but cell-impermeable metal complexes.
License
Resource Type
DOI
Date Available
Date Issued
Citation
  • Zhu, B. Z., Chao, X. J., Huang, C. H., & Li, Y. (2016). Delivering the cell-impermeable DNA ‘light-switching’Ru (ii) complexes preferentially into live-cell nucleus via an unprecedented ion-pairing method. Chemical Science, 7(7), 4016-4023. doi:10.1039/c5sc03796d
Journal Title
Journal Volume
  • 7
Journal Issue/Number
  • 7
Non-Academic Affiliation
Dichiarazione dei diritti
Funding Statement (additional comments about funding)
  • This study was supported by the Strategic Priority Research Program of CAS Grant No. XDB01020300; NSF China Grants (21207139, 21477139, 21237005, 21407163 and 21321004); and NIH Grants (ES11497, RR01008 and ES00210).
Publisher
Peer Reviewed
Language
Replaces

Le relazioni

Parents:

This work has no parents.

In Collection:

Elementi

Thumbnail Titolo Data caricata Visibilità Azioni
file details ZhuDeliveringtheCellImpermeable.pdf 10/16/2017 Pubblico
file details ZhuDeliveringtheCellImpermeableSuppInfo.pdf 10/16/2017 Pubblico
file details ZhuDeliveringtheCellImpermeableCellStructureData.cif 10/16/2017 Pubblico