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Inhibitory Effects of C₂ to C₁₀ 1-Alkynes on Ammonia Oxidation in Two Nitrososphaera Species Public Deposited

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  • A previous study showed that ammonia oxidation by the Thaumarchaeota Nitrosopumilus maritimus (group 1.1a) was resistant to concentrations of the C₈ 1-alkyne, octyne, which completely inhibits activity by ammonia-oxidizing bacteria. In this study, the inhibitory effects of octyne and other C₂ to C₁₀ 1-alkynes were evaluated on the nitrite production activity of two pure culture isolates from Thaumarchaeota group 1.1b, Nitrososphaera viennensis strain EN76 and Nitrososphaera gargensis. Both N. viennensis and N. gargensis were insensitive to concentrations of octyne that cause complete and irreversible inactivation of nitrite production by ammonia-oxidizing bacteria. However, octyne concentrations (≥20 μM) that did not inhibit N. maritimus partially inhibited nitrite production in N. viennensis and N. gargensis in a manner that did not show the characteristics of irreversible inactivation. In contrast to previous studies with an ammonia-oxidizing bacterium, Nitrosomonas europaea, octyne inhibition of N. viennensis was: (i) fully and immediately reversible, (ii) not competitive with NH₄⁺, and (iii) without effect on the competitive interaction between NH₄⁺ and acetylene. Both N. viennensis and N. gargensis demonstrated the same overall trend in regard to 1-alkyne inhibition as previously observed for N. maritimus, being highly sensitive to ≤C₅ alkynes and more resistant to longer-chain length alkynes. Reproducible differences were observed among N. maritimus, N. viennensis, and N. gargensis in regard to the extent of their resistance/sensitivity to C₆ and C₇ 1-alkynes, which may indicate differences in the ammonia monooxygenase binding and catalytic site(s) among the Thaumarchaeota.
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  • Taylor, A. E., Taylor, K., Tennigkeit, B., Palatinszky, M., Stieglmeier, M., Myrold, D. D., Schleper, C., Wagner, M., Bottomley, P. J. (2015). Inhibitory Effects of C₂ to C₁₀ 1-Alkynes on Ammonia Oxidation in Two Nitrososphaera Species. Applied and Environmental Microbiology, 81(6), 1942-1948. doi:10.1128/AEM.03688-14
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  • 81
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  • 6
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  • This research was supported by USDA NIFA award 2012-67019-3028, an Oregon Agricultural Research Foundation competitive grant, and the Agricultural Experiment Station. M.P. and M.W. were supported by a grant from the European Research Council (advanced grant NITRICARE 294343).
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