Separate Introns Gained within Short and Long Soluble Peridinin-Chlorophyll a-Protein Genes during Radiation of Symbiodinium (Dinophyceae) Clade A and B Lineages Public Deposited

http://ir.library.oregonstate.edu/concern/articles/z890rw03f

To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The article was published by the Public Library of Science and is in the public domain. The published article can be found at:  http://www.plosone.org/.

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Here we document introns in two Symbiodinium clades that were most likely gained following divergence of this genus from other peridinin-containing dinoflagellate lineages. Soluble peridinin-chlorophyll a-proteins (sPCP) occur in short and long forms in different species. Duplication and fusion of short sPCP genes produced long sPCP genes. All short and long sPCP genes characterized to date, including those from free living species and Symbiodinium sp. 203 (clade C/type C2) are intronless. However, we observed that long sPCP genes from two Caribbean Symbiodinium clade B isolates each contained two introns. To test the hypothesis that introns were gained during radiation of clade B, we compared sPCP genomic and cDNA sequences from 13 additional distinct Caribbean and Pacific Symbiodinium clade A, B, and F isolates. Long sPCP genes from all clade B/B1 and B/B19 descendants contain orthologs of both introns. Short sPCP genes from S. pilosum (A/A2) and S. muscatinei (B/B4) plus long sPCP genes from S. microadriaticum (A/A1) and S. kawagutii (F/F1) are intronless. Short sPCP genes of S. microadriaticum have a third unique intron. Symbiodinium clade B long sPCP sequences are useful for assessing divergence among B1 and B19 descendants. Phylogenetic analyses of coding sequences from four dinoflagellate orders indicate that introns were gained independently during radiation of Symbiodinium clades A and B. Long sPCP introns were present in the most recent common ancestor of Symbiodinium clade B core types B1 and B19, which apparently diverged sometime during the Miocene. The clade A short sPCP intron was either gained by S. microadriaticum or possibly by the ancestor of Symbiodinium types A/A1, A3, A4 and A5. The timing of short sPCP intron gain in Symbiodinium clade A is less certain. But, all sPCP introns were gained after fusion of ancestral short sPCP genes, which we confirm as occurring once in dinoflagellate evolution.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Reichman, J. R., Vize, P. D. (2014). Separate Introns Gained within Short and Long Soluble Peridinin-Chlorophyll a-Protein Genes during Radiation of Symbiodinium (Dinophyceae) Clade A and B Lineages. PLoS ONE, 9(10), e110608. doi:10.1371/journal.pone.0110608
Series
Rights Statement
Funding Statement (additional comments about funding)
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2014-12-23T20:43:15Z No. of bitstreams: 3 license_rdf: 1089 bytes, checksum: 0a703d871bf062c5fdc7850b1496693b (MD5) ReichmanJayBotanyPlantPathologySeparateIntronsGained.pdf: 1193650 bytes, checksum: d4708584897c1b618839b0722205004b (MD5) ReichmanJayBotanyPlantPathologySeparateIntronsGained_SupportingInformation.zip: 4772634 bytes, checksum: 18fd793fe8eb565f1ad06c65aba98bd9 (MD5)
  • description.provenance : Made available in DSpace on 2014-12-23T20:43:34Z (GMT). No. of bitstreams: 3 license_rdf: 1089 bytes, checksum: 0a703d871bf062c5fdc7850b1496693b (MD5) ReichmanJayBotanyPlantPathologySeparateIntronsGained.pdf: 1193650 bytes, checksum: d4708584897c1b618839b0722205004b (MD5) ReichmanJayBotanyPlantPathologySeparateIntronsGained_SupportingInformation.zip: 4772634 bytes, checksum: 18fd793fe8eb565f1ad06c65aba98bd9 (MD5) Previous issue date: 2014-10-17
  • description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2014-12-23T20:43:34Z (GMT) No. of bitstreams: 3 license_rdf: 1089 bytes, checksum: 0a703d871bf062c5fdc7850b1496693b (MD5) ReichmanJayBotanyPlantPathologySeparateIntronsGained.pdf: 1193650 bytes, checksum: d4708584897c1b618839b0722205004b (MD5) ReichmanJayBotanyPlantPathologySeparateIntronsGained_SupportingInformation.zip: 4772634 bytes, checksum: 18fd793fe8eb565f1ad06c65aba98bd9 (MD5)

Relationships

In Administrative Set:
Last modified: 07/26/2017 Default
Citations:

EndNote | Zotero | Mendeley

Items