Three-Dimensional Reflectance Traction Microscopy Public Deposited

http://ir.library.oregonstate.edu/concern/articles/zk51vj48k

Data Availability Statement: All relevant data are available within the paper and on GitHub at https:// github.com/bosunorst/Partial-Volume-Correlation/.

This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by the Public Library of Science. The published article can be found at:  http://journals.plos.org/plosone/

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Cells in three-dimensional (3D) environments exhibit very different biochemical and biophysical phenotypes compared to the behavior of cells in two-dimensional (2D) environments. As an important biomechanical measurement, 2D traction force microscopy can not be directly extended into 3D cases. In order to quantitatively characterize the contraction field, we have developed 3D reflectance traction microscopy which combines confocal reflection imaging and partial volume correlation postprocessing. We have measured the deformation field of collagen gel under controlled mechanical stress. We have also characterized the deformation field generated by invasive breast cancer cells of different morphologies in 3D collagen matrix. In contrast to employ dispersed tracing particles or fluorescently-tagged matrix proteins, our methods provide a label-free, computationally effective strategy to study the cell mechanics in native 3D extracellular matrix.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Kim, J., Jones, C. A., Groves, N. S., & Sun, B. (2016). Three-Dimensional Reflectance Traction Microscopy. PLoS ONE, 11(6), e0156797. doi:10.1371/journal.pone.0156797
Series
Rights Statement
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2016-07-15T14:10:24Z (GMT). No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) KimThreeDimensionalReflectance.PDF: 8853778 bytes, checksum: 5c539868435c1b4551b05ebfd975757a (MD5) KimThreeDimensionalReflectanceSupportingInformation.zip: 4647351 bytes, checksum: b31f47cf89e583af0b8d9cfb8944773a (MD5) Previous issue date: 2016-06-15
  • description.provenance : Submitted by Patricia Black (patricia.black@oregonstate.edu) on 2016-07-15T14:09:56Z No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) KimThreeDimensionalReflectance.PDF: 8853778 bytes, checksum: 5c539868435c1b4551b05ebfd975757a (MD5) KimThreeDimensionalReflectanceSupportingInformation.zip: 4647351 bytes, checksum: b31f47cf89e583af0b8d9cfb8944773a (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2016-07-15T14:10:24Z (GMT) No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) KimThreeDimensionalReflectance.PDF: 8853778 bytes, checksum: 5c539868435c1b4551b05ebfd975757a (MD5) KimThreeDimensionalReflectanceSupportingInformation.zip: 4647351 bytes, checksum: b31f47cf89e583af0b8d9cfb8944773a (MD5)

Relationships

In Administrative Set:
Last modified: 07/26/2017 Default
Citations:

EndNote | Zotero | Mendeley

Items