Article

 

Three-Dimensional Reflectance Traction Microscopy Öffentlichkeit Deposited

Herunterladbarer Inhalt

PDF Herunterladen
https://ir.library.oregonstate.edu/concern/articles/zk51vj48k

Descriptions

Attribute NameValues
Creator
Abstract
  • Cells in three-dimensional (3D) environments exhibit very different biochemical and biophysical phenotypes compared to the behavior of cells in two-dimensional (2D) environments. As an important biomechanical measurement, 2D traction force microscopy can not be directly extended into 3D cases. In order to quantitatively characterize the contraction field, we have developed 3D reflectance traction microscopy which combines confocal reflection imaging and partial volume correlation postprocessing. We have measured the deformation field of collagen gel under controlled mechanical stress. We have also characterized the deformation field generated by invasive breast cancer cells of different morphologies in 3D collagen matrix. In contrast to employ dispersed tracing particles or fluorescently-tagged matrix proteins, our methods provide a label-free, computationally effective strategy to study the cell mechanics in native 3D extracellular matrix.
License
Resource Type
DOI
Date Available
Date Issued
Citation
Journal Title
Journal Volume
  • 11
Journal Issue/Number
  • 6
Academic Affiliation
Urheberrechts-Erklärung
Related Items
Publisher
Peer Reviewed
Language
Replaces

Beziehungen

Parents:

This work has no parents.

Artikel