Genes affecting novel seed constituents in Limnanthes alba Benth: transcriptome analysis of developing embryos and a new genetic map of meadowfoam Public Deposited

http://ir.library.oregonstate.edu/concern/articles/zk51vj58t

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  • The seed oil of meadowfoam, a new crop in the Limnanthaceae family, is highly enriched in very long chain fatty acids that are desaturated at the Δ5 position. The unusual oil is desirable for cosmetics and innovative industrial applications and the seed meal remaining after oil extraction contains glucolimnanthin, a methoxylated benzylglucosinolate whose degradation products are herbicidal and anti-microbial. Here we describe EST analysis of the developing seed transcriptome that identified major genes involved in biosynthesis and assembly of the seed oil and in glucosinolate metabolic pathways. mRNAs encoding acyl-CoA Δ5 desaturase were notably abundant. The library was searched for simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs). Fifty-four new SSR markers and eight candidate gene markers were developed and combined with previously developed SSRs to construct a new genetic map for Limnanthes alba. Mapped genes in the lipid biosynthetic pathway encode 3-ketoacyl-CoA synthase (KCS), Δ5 desaturase (Δ5DS), lysophosphatidylacyl-acyl transferase (LPAT), and acyl-CoA diacylglycerol acyl transferase (DGAT). Mapped genes in glucosinolate biosynthetic and degradation pathways encode CYP79A, myrosinase (TGG), and epithiospecifier modifier protein (ESM). The resources developed in this study will further the domestication and improvement of meadowfoam as an oilseed crop.
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  • Slabaugh, M. B., Cooper, L. D., Kishore, V. K., Knapp, S. J., & Kling, J. G. (2015). Genes affecting novel seed constituents in Limnanthes alba Benth: transcriptome analysis of developing embryos and a new genetic map of meadowfoam. PeerJ, 3, e915. doi:10.7717/peerj.915
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  • description.provenance : Submitted by Patricia Black (patricia.black@oregonstate.edu) on 2015-08-03T16:58:29Z No. of bitstreams: 8 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) SlabaughMaryCropSoilSciGenesAffectingNovel.pdf: 975191 bytes, checksum: 52083083144d675ab9db70304d42cf67 (MD5) SlabaughMaryCropSoilSciGenesAffectingNovelSupp1Figure1.pdf: 122562 bytes, checksum: 443490fee9fa6250409af92ba0fb2354 (MD5) SlabaughMaryCropSoilSciGenesAffectingNovelSupp2TableS1.xls: 2633728 bytes, checksum: a3faa77071d343588c2553177739caf3 (MD5) SlabaughMaryCropSoilSciGenesAffectingNovelSupp3TableS2.pdf: 352549 bytes, checksum: 2751537b816e75abcc1723437329743a (MD5) SlabaughMaryCropSoilSciGenesAffectingNovelSupp4TableS3.xls: 62976 bytes, checksum: eb9a4913015c15377d3273239c12b9d9 (MD5) SlabaughMaryCropSoilSciGenesAffectingNovelSupp5TableS4.xls: 26112 bytes, checksum: c30a382352b9e5ce812ebac470aac534 (MD5) SlabaughMaryCropSoilSciGenesAffectingNovelSupp6TableS5.xls: 45568 bytes, checksum: f3a0d72e56180831ca8e9acad41cbc46 (MD5)
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  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2015-08-03T16:58:58Z (GMT) No. of bitstreams: 8 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) SlabaughMaryCropSoilSciGenesAffectingNovel.pdf: 975191 bytes, checksum: 52083083144d675ab9db70304d42cf67 (MD5) SlabaughMaryCropSoilSciGenesAffectingNovelSupp1Figure1.pdf: 122562 bytes, checksum: 443490fee9fa6250409af92ba0fb2354 (MD5) SlabaughMaryCropSoilSciGenesAffectingNovelSupp2TableS1.xls: 2633728 bytes, checksum: a3faa77071d343588c2553177739caf3 (MD5) SlabaughMaryCropSoilSciGenesAffectingNovelSupp3TableS2.pdf: 352549 bytes, checksum: 2751537b816e75abcc1723437329743a (MD5) SlabaughMaryCropSoilSciGenesAffectingNovelSupp4TableS3.xls: 62976 bytes, checksum: eb9a4913015c15377d3273239c12b9d9 (MD5) SlabaughMaryCropSoilSciGenesAffectingNovelSupp5TableS4.xls: 26112 bytes, checksum: c30a382352b9e5ce812ebac470aac534 (MD5) SlabaughMaryCropSoilSciGenesAffectingNovelSupp6TableS5.xls: 45568 bytes, checksum: f3a0d72e56180831ca8e9acad41cbc46 (MD5)

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