Alternative splicing in the obligate biotrophic oomycete pathogen, Pseudoperonospora cubensis Public Deposited

http://ir.library.oregonstate.edu/concern/datasets/c534fv018

Suggested citation: "Burkhardt, Alyssa; Buchanan, Alex; Cumbie, Jason S.; Savory, Elizabeth, A.; Chang, Jeff H.; Day, Brad (2014): Alternative splicing in the obligate biotrophic oomycete pathogen, Pseudoperonospora cubensis. Oregon State University Libraries. Dataset.  http://dx.doi.org/10.7267/N9TD9V7M"

See related paper: "Burkhardt, Alyssa, Alex Buchanan, Jason S Cumbie, Elizabeth A Savory, Jeff Chang, and Brad Day (2014) Alternative Splicing in the Obligate Biotrophic Oomycete Pathogen, Pseudoperonospora Cubensis. Molecular Plant-Microbe Interactions [issue forthcoming] doi:10.1094/MPMI-09-14-0300-FI."

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  • We re-sequenced the Pseudoperonospora cubensis transcriptome to provide the first extensive transcriptome-wide survey of alternative splicing in an obligate biotrophic pathogen during host infection. The libraries from biological replicates of cucumber leaves infected with Ps. cubensis for 2, 3, 4, and 8 days post inoculation (dpi) were re-sequenced using 100-mer paired-end (PE) sequencing on four channels of an Illumina HiSeq. As a first step in the characterization of alternative splicing, we used the RNA-Seq reads to reannotate the Ps. cubensis genome, which is deposited as a gff file, along with a relational table that associates the most current annotation to the previous Ps. cubensis annotation.
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  • Burkhardt, Alyssa; Buchanan, Alex; Cumbie, Jason S.; Savory, Elizabeth, A.; Chang, Jeff H.; Day, Brad (2014): Alternative splicing in the obligate biotrophic oomycete pathogen, Pseudoperonospora cubensis. Oregon State University Libraries. Dataset. http://dx.doi.org/10.7267/N9TD9V7M
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  • description.provenance : Submitted by Amanda Whitmire (amanda.whitmire@oregonstate.edu) on 2014-06-05T17:44:41ZNo. of bitstreams: 2license_rdf: 1089 bytes, checksum: 0a703d871bf062c5fdc7850b1496693b (MD5)placeholder.txt: 224 bytes, checksum: bf2bb61d78831061bb1258faf048e778 (MD5)
  • description.provenance : Approved for entry into archive by Amanda Whitmire(amanda.whitmire@oregonstate.edu) on 2014-06-05T17:48:46Z (GMT) No. of bitstreams: 2license_rdf: 1089 bytes, checksum: 0a703d871bf062c5fdc7850b1496693b (MD5)placeholder.txt: 224 bytes, checksum: bf2bb61d78831061bb1258faf048e778 (MD5)
  • description.provenance : Made available in DSpace on 2014-06-05T17:48:46Z (GMT). No. of bitstreams: 2license_rdf: 1089 bytes, checksum: 0a703d871bf062c5fdc7850b1496693b (MD5)placeholder.txt: 224 bytes, checksum: bf2bb61d78831061bb1258faf048e778 (MD5)

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