Characterization of Neurospora crassa “Fatty Acid Regulator” transcription factors, FAR-1 and FAR-2, by ChIP- and RNA-sequencing Public Deposited

http://ir.library.oregonstate.edu/concern/datasets/sf268993z

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  • Transcription factor (TF) genes were modified endogenously to include epitope tags for identification of TF protein binding sites by Chromatin Immunoprecipitation (ChIP), followed by high throughput sequencing. We used RNA-sequencing in carbon sources of sucrose, butyrate, and oleate in far-1, far-2, and a double far-1; far-2 mutant to find transcripts influenced by FAR regulation. This dataset includes (1) ChIP peaks indicative of genomic binding locations of FAR-1 and FAR-2 (4 files), (2) analysis of gene ontology (GO) by two methods (4 files), and (3) total RNA-sequencing for wild type, delta-far-1, delta-far-2, and delta-far-1; delta-far-2 mutants, with differential expression as evaluated by CuffDiff (2 files).
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  • Bredeweg, Erin L.; Freitag, Michael (2014): Characterization of Neurospora crassa “Fatty Acid Regulator” transcription factors, FAR-1 and FAR-2, by ChIP- and RNA-sequencing. Oregon State University. Dataset. http://dx.doi.org/10.7267/N9BG2KWH
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