Characterization of Neurospora crassa “Fatty Acid Regulator” transcription factors, FAR-1 and FAR-2, by ChIP- and RNA-sequencing Public Deposited

http://ir.library.oregonstate.edu/concern/datasets/sf268993z

This dataset is not available to the public until March 2016. Suggested citation: "Bredeweg, Erin L.; Freitag, Michael (2014): Characterization of Neurospora crassa “Fatty Acid Regulator” transcription factors, FAR-1 and FAR-2, by ChIP- and RNA-sequencing. Oregon State University Libraries. Dataset.  http://dx.doi.org/10.7267/N9BG2KWH" See related Ph.D. dissertation at:  http://hdl.handle.net/1957/46468.

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Transcription factor (TF) genes were modified endogenously to include epitope tags for identification of TF protein binding sites by Chromatin Immunoprecipitation (ChIP), followed by high throughput sequencing. We used RNA-sequencing in carbon sources of sucrose, butyrate, and oleate in far-1, far-2, and a double far-1; far-2 mutant to find transcripts influenced by FAR regulation. This dataset includes (1) ChIP peaks indicative of genomic binding locations of FAR-1 and FAR-2 (4 files), (2) analysis of gene ontology (GO) by two methods (4 files), and (3) total RNA-sequencing for wild type, delta-far-1, delta-far-2, and delta-far-1; delta-far-2 mutants, with differential expression as evaluated by CuffDiff (2 files).
License
Resource Type
Date Available
Date Issued
Citation
  • Bredeweg, Erin L.; Freitag, Michael (2014): Characterization of Neurospora crassa “Fatty Acid Regulator” transcription factors, FAR-1 and FAR-2, by ChIP- and RNA-sequencing. Oregon State University Libraries. Dataset. http://dx.doi.org/10.7267/N9BG2KWH
Keyword
Rights Statement
Funding Statement (additional comments about funding)
Peer Reviewed
Language
Replaces

Relationships

In Administrative Set:
Last modified: 01/23/2018 Default
Citations:

EndNote | Zotero | Mendeley

Items