Dataset

Characterization of Neurospora crassa “Fatty Acid Regulator” transcription factors, FAR-1 and FAR-2, by ChIP- and RNA-sequencing Public Deposited

Analytics

Citeable URL: https://ir.library.oregonstate.edu/concern/datasets/sf268993z

Descriptions

Attribute Name	Values
Creator	Bredeweg, Erin L. Freitag, Michael
Abstract	Transcription factor (TF) genes were modified endogenously to include epitope tags for identification of TF protein binding sites by Chromatin Immunoprecipitation (ChIP), followed by high throughput sequencing. We used RNA-sequencing in carbon sources of sucrose, butyrate, and oleate in far-1, far-2, and a double far-1; far-2 mutant to find transcripts influenced by FAR regulation. This dataset includes (1) ChIP peaks indicative of genomic binding locations of FAR-1 and FAR-2 (4 files), (2) analysis of gene ontology (GO) by two methods (4 files), and (3) total RNA-sequencing for wild type, delta-far-1, delta-far-2, and delta-far-1; delta-far-2 mutants, with differential expression as evaluated by CuffDiff (2 files).
License	CC0 1.0 Universal
Resource Type	Dataset
DOI	https://doi.org/10.7267/N9BG2KWH
Date Available	2017-03-01T09:00:08+00:00
Date Issued	2014-01-06
Citation	Bredeweg, Erin L.; Freitag, Michael (2014): Characterization of Neurospora crassa “Fatty Acid Regulator” transcription factors, FAR-1 and FAR-2, by ChIP- and RNA-sequencing. Oregon State University. Dataset. http://dx.doi.org/10.7267/N9BG2KWH
Keyword	cutinase transcription factor transcription factor network glyoxylate cycle lipase fatty acid regulator beta-oxidation
Rights Statement	No Copyright - United States
Related Items	See related Ph.D. dissertation.
Funding Statement (additional comments about funding)	This work is part of an investigation funded by Functional Genomics and Systems Biology (NcFGSB) program project funded by the NIH (PO1GM).
Peer Reviewed	No
Language	English [eng]
Replaces	http://hdl.handle.net/1957/44860
Embargo reason	Ongoing Research
Embargo date range	2018-11-05 to 2019-12-06

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Items

Thumbnail	Title	Date Uploaded	Visibility	Actions
	TableS01_FAR1S_ChIP.xlsx	12/04/2017	Public
	TableS12_genes_fpkm_tracking.txt	12/04/2017	Public
	TableS05_FAR2B_ChIP.xlsx	12/04/2017	Public
	TableS04_FAR2S_ChIP.xlsx	12/04/2017	Public
	TableS06_FAR2O_ChIP.xlsx	12/04/2017	Public
	Metadata_FARs.docx	12/04/2017	Public
	TableS02_FAR1B_ChIP.xlsx	12/04/2017	Public
	TableS03_FAR1O_ChIP.xlsx	12/04/2017	Public
	TableS07_FAR1-GO_ChIP.xlsx	12/04/2017	Public
	TableS08_FAR2-GO_ChIP.xlsx	12/04/2017	Public