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Characterization of Neurospora crassa “Fatty Acid Regulator” transcription factors, FAR-1 and FAR-2, by ChIP- and RNA-sequencing

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https://ir.library.oregonstate.edu/concern/datasets/sf268993z

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Abstract
  • Transcription factor (TF) genes were modified endogenously to include epitope tags for identification of TF protein binding sites by Chromatin Immunoprecipitation (ChIP), followed by high throughput sequencing. We used RNA-sequencing in carbon sources of sucrose, butyrate, and oleate in far-1, far-2, and a double far-1; far-2 mutant to find transcripts influenced by FAR regulation. This dataset includes (1) ChIP peaks indicative of genomic binding locations of FAR-1 and FAR-2 (4 files), (2) analysis of gene ontology (GO) by two methods (4 files), and (3) total RNA-sequencing for wild type, delta-far-1, delta-far-2, and delta-far-1; delta-far-2 mutants, with differential expression as evaluated by CuffDiff (2 files).
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Citation
  • Bredeweg, Erin L.; Freitag, Michael (2014): Characterization of Neurospora crassa “Fatty Acid Regulator” transcription factors, FAR-1 and FAR-2, by ChIP- and RNA-sequencing. Oregon State University. Dataset. http://dx.doi.org/10.7267/N9BG2KWH
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Related Items
  • See related Ph.D. dissertation.
Funding Statement (additional comments about funding)
  • This work is part of an investigation funded by Functional Genomics and Systems Biology (NcFGSB) program project funded by the NIH (PO1GM).
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Embargo reason
  • Ongoing Research
Embargo date range
  • 2018-11-05 to 2019-12-06

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Items

Thumbnail Title Date Uploaded Visibility Actions
file details TableS09_MIPS-FAR1-RNAfiltered.xlsx 12/04/2017 Public
file details TableS10_MIPS-FAR2-RNAfiltered.xlsx 12/04/2017 Public
file details TableS11_gene_differential-expression.txt 12/04/2017 Public