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Influence of iron concentration on the expression of the ferripyoverdine receptor gene FpvAIIb of Pseudomonas aeruginosa Public Deposited

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  • Pseudomonas aeruginosa is an environmental bacterium which opportunistically infects humans. Commonly residing in irondepleted environments, P. aeruginosa uses iron-chelating molecules called siderophores to scavenge iron from its environment. The most prevalent siderophore used by P. aeruginosa is known as pyoverdine. After the cell synthesizes and secretes pyoverdine, the molecule binds with high affinity to iron in the environment. The iron-pyoverdine (ferripyoverdine) complex is brought into the cell upon binding to the ferripyoverdine receptor FpvA, and the iron is used to carry out critical cell functions. Previous studies in our lab have used pyocin S3, which is a protein toxin that enters target cells through a sub-type of the ferripyoverdine receptor called FpvAIIb in P. aeruginosa ATCC 27853. These studies have shown that pyocin S3 is able to kill cells independently of the iron concentration in the medium, suggesting that the FpvAIIb receptor is expressed equally in different iron concentrations. This would be a novel expression pattern for FpvA, which is currently known to be inversely regulated with iron availability in the strain PAO1. The aim of this research study is to determine whether P. aeruginosa ATCC 27853 expresses the FpvAIIb gene in an iron-independent manner.
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