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Development of a Standardized DNA Database for Chinook Salmon Public Deposited

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  • An international multi-laboratory project was conducted to develop a standardized DNA database for Chinook salmon (Oncorhynchus tshawytscha). This project was in response to the needs of the Chinook Technical Committee of the Pacific Salmon Commission to identify stock composition of Chinook salmon caught in fisheries during their oceanic migrations. Nine genetics laboratories identified 13 microsatellite loci that could be reproducibly assayed in each of the laboratories. To test that the loci were reproducible among laboratories, blind tests were conducted to verify scoring consistency for the nearly 500 total alleles. Once standardized, a dataset of over 16,000 Chinook salmon representing 110 putative populations was constructed ranging throughout the area of interest of the Pacific Salmon Commission from Southeast Alaska to the Sacramento River in California. The dataset differentiates the major known genetic lineages of Chinook salmon and provides a tool for genetic stock identification of samples collected from mixed fisheries. A diverse group of scientists representing the disciplines of fishery management, genetics, fishery administration, population dynamics, and sampling theory are now developing recommendations for the integration of these genetic data into ocean salmon management.Original Abstract: Se realizo un proyecto internacional con la participation de diversos laboratorios con la finalidad de desarrollar una base de datos estandarizada de DNA para el salmon rey (Oncorhynchus tshawytscha). Dicho proyecto surgi6 como respuesta a las necesidades del Comite Tecnico Chinook de la Comision del Salmon del Pacifico para identificar la composition poblacional del salmon rey que es capturado por la pesqueria durante su migration. Un total de nueve laboratorios de analisis geneticos identificaron y reprodujeron cada uno 13 loci microsatelites. Con el objeto de probar que dichos loci fueran reproducibles entre laboratorios, se condujeron pruebas anonimas para verificar la consistencia de casi 500 alelos. Una vez estandarizada, se construyo una base de datos construida con information proveniente de mas de 16,000 salmones que representan 110 poblaciones putativas distribuidas a lo largo del area de interes de la Comision del Salm6n del Pacifico, del sureste de Alaska hasta el Rio Sacramento, California. La base de datos sirve tanto para identificar gene'ticamente los distintos stocks de salmon rey a partir de muestras combinadas provenientes de la pesqueria como para diferenciar el linaje genetico conocido mas importante de esta especie. En la actualidad, un importante grupo de cientificos especializados en disciplinas como el manejo y administracion de pesquerias, genetica, dinamica poblacional y teoria del muestreo estan desarrollando recomendaciones para que esta base de datos geneticos se incorpore en el manejo del salmon.
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  • Seeb, L. W., Antonovich, A., Banks, M. A., Beacham, T. D., Bellinger, M. R., Blankenship, S. M., & Campbell, M. R. (2007, November). Development of a standardized DNA database for Chinook salmon. Fisheries, 32(11), 540-552.
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  • Primary funding for the study was provided by the U.S. Department of Commerce, NOAA, NMFS, funds appropriated to fund the technical work required for the U.S. section of the Pacific Salmon Treaty to implement the Chinook Salmon Agreement regarding abundance based management. Partial funding for the U.S. Fish and Wildlife Service was provided by the U.S. Yukon River Research and Management Fund (USRM-08-05) and to the University of Washington by Joint Institute for the Study of the Atmosphere and Ocean (JISAO) under NOAA Cooperative Agreement NA17RJ1232, Contribution 1444. Significant matching funds were provided by all participating institutions.
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  • description.provenance : Submitted by Judy Mullen (judy.mullen@oregonstate.edu) on 2011-09-19T23:40:42Z No. of bitstreams: 1 DevelopmentOfAStandardized DNA.pdf: 456833 bytes, checksum: 86477d228432f4e1bd7f7712e2d8d620 (MD5)
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