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Improving in vitro mineral nutrition for diverse pear germplasm

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https://ir.library.oregonstate.edu/concern/articles/2f75r866x

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Abstract
  • Mineral nutrition in the media used for growth of in vitro plants is often difficult to optimize due to complex chemical interactions of required nutrients. The response of plant tissue to standard growth media varies widely due to the genetic diversity of the plant species studied. This study was designed as the initial step in determining the optimal mineral nutrient requirements for micropropagation of shoot tips from a collection of genetically diverse pear germplasm. Five mineral nutrient factors were defined from Murashige and Skoog salts: NH₄NO₃, KNO₃, mesos (CaCl₂•2H₂0-KH₂PO₄-MgSO₄), micronutrients (B, Cu, Co, I, Mn, Mo, and Zn), and Fe-EDTA. Each factor was varied over a range of concentrations. Treatment combinations were selected using response surface methods. Five pears in three species (Pyrus communis 'Horner 51,' 'Old Home x Farmingdale 87,' 'Winter Nelis,' Pyrus dimorphophylla, and Pyrus ussuriensis 'Hang Pa Li') were grown on each treatment combination, responses were measured, and each response was analyzed by analysis of variance. The analyses resulted in the identification of the following factors with the single largest effects on plant response: shoot quality (mesos), leaf spotting/necrosis (mesos), leaf size (mesos), leaf color (mesos, NH₄NO₃, and KNO₃), shoot number (NH₄NO₃ and Fe), nodes (NH₄NO₃ and KNO₃), and shoot length (mesos and Fe). Factors with the largest effects (mesos and Fe) were similar among the genotypes. This approach was very successful for defining the appropriate types and concentrations of mineral nutrients for micropropagation of diverse pear genotypes.
  • Keywords: Surface response design, Growth medium, Mineral nutrition, Pyrus . Micropropagation
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  • Reed, B. M., Wada, S., DeNoma, J., & Niedz, R. P. (2013). Improving in vitro mineral nutrition for diverse pear germplasm. In Vitro Cellular & Developmental Biology - Plant, 49(3), 343-355. doi:10.1007/s11627-013-9504-1
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  • 49
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  • 3
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  • This project was funded by a grant from the Oregon Association of Nurseries and the Oregon Department of Agriculture and by United States Department of Agriculture-Agricultural Research Service CRIS project 5358-21000- 0-38-00D.
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