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An Introduced Snail and Spread of Liver Flukes in Oregon Public Deposited

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  • • Objective: Investigate the invasive snail Lymnaea auricularia as a potential intermediate host of Fasciola hepatica in Oregon • Sample: 330 Lymnaea auricularia snails collected from a farm in Eugene, Oregon from May 2018 – August 2018 • Procedures: L. auricularia snails were plated in petri dishes, left overnight, and examined the next day under a dissecting scope for emergence of cercariae. Cercariae that were found were examined under a microscope for morphologic features consistent with Fasciola hepatica, photographed, and preserved in ethanol. Snails were then euthanized via freezing, homogenized, and analyzed for Fasciola hepatica DNA using PCR • Results: Fasciola hepatica cercariae were not identified in Lymnaea auricularia snails with either microscopy or PCR. • Conclusions and Clinical Relevance: This project did not identify Lymnaea auricularia as a carrier of Fasciola hepatica in Oregon. However, complications such as timing of cercarial shedding and presence of uninvestigated water sources on the farm may have precluded discovery of fluke cercariae in these snails. Further investigation of L. auricularia as a potential carrier of Fasciola hepatica will help address the broader goal of tracking intermediate hosts of Fasciola hepatica and enabling better management of Fascioliasis.
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