Adherence of Clostridium difficile spores to Caco-2 cells in culture Public Deposited

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This is the author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Society for General Microbiology and can be found at:  http://jmm.sgmjournals.org/.

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  • Clostridium difficile is the causative agent of the majority of antibiotic associated diarrheas. C. difficile spores are recognized as the morphotype of transmission, infection and persistence. However, there is a lack of knowledge on how C. difficile spores interact with the host’s epithelial surfaces. In this context, we have characterized the ability of C. difficile spores to adhere to human Caco-2 cells. Despite the similarities in spore-surface hydrophobicity between spores of C. difficile and C. perfringens (another enteric pathogen that also sporulates in the gut), spores of C. difficile adhere better to Caco-2 cells. Adherence to Caco-2 cells was significantly reduced when C. difficile spores were trypsin-treated. Sonication of C. difficile spores altered the ultrastructure of the outermost exosporium-like structure, released two protein species of ~ 40-kDa and significantly reduced spore-hydrophobicity and adherence to Caco-2 cells. Using a trifunctional crosslinker, we were able to co-immunoprecipitate four protein species from the surface of Caco-2 cells. In conclusion, this study provides, for the first time evidence that C. difficile spores adhere to human intestinal enterocyte-like cells through spore- and enterocytic surface specific ligand(s) and/or receptor(s).
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  • Paredes-Sabja, D., & Sarker, M. R. (2012). Adherence of clostridium difficile spores to caco-2 cells in culture. Journal of Medical Microbiology, 61(Pt 9), 1208-1218. doi: 10.1099/jmm.0.043687-0
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  • description.provenance : Made available in DSpace on 2013-02-22T18:41:29Z (GMT). No. of bitstreams: 1 SarkerMahfuzurMicrobiologyAdherenceClostridiumDifficile.pdf: 484773 bytes, checksum: 37ae71140c871bf17e2d389c8d8421ff (MD5) Previous issue date: 2012-09
  • description.provenance : Approved for entry into archive by Deanne Bruner(deanne.bruner@oregonstate.edu) on 2013-02-22T18:41:29Z (GMT) No. of bitstreams: 1 SarkerMahfuzurMicrobiologyAdherenceClostridiumDifficile.pdf: 484773 bytes, checksum: 37ae71140c871bf17e2d389c8d8421ff (MD5)
  • description.provenance : Submitted by Deanne Bruner (deanne.bruner@oregonstate.edu) on 2013-02-22T18:36:57Z No. of bitstreams: 1 SarkerMahfuzurMicrobiologyAdherenceClostridiumDifficile.pdf: 484773 bytes, checksum: 37ae71140c871bf17e2d389c8d8421ff (MD5)

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