Recombineering and stable integration of the Pseudomonas syringae pv. syringae 61 hrp/hrc cluster into the genome of the soil bacterium Pseudomonas fluorescens Pf0-1 Public Deposited

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  • SUMMARY Many Gram-negative bacteria use a type III secretion system (T3SS) to establish associations with their hosts. The T3SS is a conduit for direct injection of type-III effector proteins into host cells, where they manipulate the host for the benefit of the infecting bacterium. For plant-associated pathogens, the variations in number and amino acid sequences of type-III effectors, as well as their functional redundancy, make studying type-III effectors challenging. To mitigate this challenge, we developed a stable delivery system for individual or defined sets of type-III effectors into plant cells. We used recombineering and Tn5-mediated transposition to clone and stably integrate, respectively, the complete hrp/hrc region from Pseudomonas syringae pv. syringae 61 into the genome of the soil bacterium Pseudomonas fluorescens Pf0-1. We describe our development of Effector-to-Host Analyzer (EtHAn), and demonstrate its utility for studying effectors for their in planta functions.
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  • William J. Thomas1,2,†, Caitlin A. Thireault1,†, Jeffrey A. Kimbrel1,2 and Jeff H. Chang1,2,3,* (2009) Recombineering and stable integration of the Pseudomonas syringae pv. syringae 61 hrp/hrc cluster into the genome of the soil bacterium Pseudomonas fluorescens Pf0-1 The Plant Journal doi: 10.1111/j.1365-313X.2009.03998.x
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  • description.provenance : Made available in DSpace on 2010-09-14T17:08:35Z (GMT). No. of bitstreams: 1 Cait Thireault Plant J 2009 Thomas19682294_.pdf: 402793 bytes, checksum: 0c51321d8cd464fe4176e8d36dca721a (MD5) Previous issue date: 2009-08-06
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  • description.provenance : Approved for entry into archive by Michael Boock(michael.boock@oregonstate.edu) on 2010-09-14T17:08:35Z (GMT) No. of bitstreams: 1 Cait Thireault Plant J 2009 Thomas19682294_.pdf: 402793 bytes, checksum: 0c51321d8cd464fe4176e8d36dca721a (MD5)

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