Distinct Argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline Public Deposited

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  • Endogenous small RNAs (endo-siRNAs) interact with Argonaute (AGO) proteins to mediate sequence-specific regulation of diverse biological processes. Here, we combine deep-sequencing and genetic approaches to explore the biogenesis and function of endo-siRNAs in C. elegans. We describe conditional alleles of the dicer-related helicase, drh-3, that abrogate both RNA interference and the biogenesis of endo-siRNAs, called 22G-RNAs. DRH-3 is a core component of RNA-dependent RNA polymerase (RdRP) complexes essential for several distinct 22G-RNA systems. We show that in the germ-line, one system is dependent on worm-specific AGOs, including WAGO-1, which localizes to germ-line nuage structures called P-granules. WAGO-1 silences certain genes, transposons, pseudogenes and cryptic loci. Finally, we demonstrate that components of the nonsense-mediated decay pathway function in at least one WAGO-mediated surveillance pathway. These findings broaden our understanding of the biogenesis and diversity of 22G-RNAs and suggest novel regulatory functions for small RNAs.
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  • Gu, W., Shirayama, M., Conte, D. Jr., Vasale, J., Batista, P. J., Claycomb, J. M., Moresco, J. J., Youngman, E. M., Keys, J., Stoltz, M. J., Chen, C. C., Chaves, D. A., Duan, S., Kasschau, K. D., Fahlgren, N., Yates 3rd, J. R., Mitani, S., Carrington, J. C., Mello, C. C. (2010). Distinct Argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline. Molecular Cell, 36(2), 231-244
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  • description.provenance : Submitted by Mary Phan (mpscanner@gmail.com) on 2011-01-31T18:19:19Z No. of bitstreams: 1 nihms-150534.pdf: 2182068 bytes, checksum: f8397a7671e270c92c14f7c77b1e1521 (MD5)
  • description.provenance : Made available in DSpace on 2011-02-01T20:39:49Z (GMT). No. of bitstreams: 1 nihms-150534.pdf: 2182068 bytes, checksum: f8397a7671e270c92c14f7c77b1e1521 (MD5) Previous issue date: 2010-10-23
  • description.provenance : Approved for entry into archive by Michael Boock(michael.boock@oregonstate.edu) on 2011-02-01T20:39:49Z (GMT) No. of bitstreams: 1 nihms-150534.pdf: 2182068 bytes, checksum: f8397a7671e270c92c14f7c77b1e1521 (MD5)

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