Accelerated Identification of Proteins by Mass Spectrometry by Employing Covalent Pre-Gel Staining with Uniblue A Public Deposited

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  • Background: The identification of proteins by mass spectrometry is a standard method in biopharmaceutical quality control and biochemical research. Prior to identification by mass spectrometry, proteins are usually pre-separated by electrophoresis. However, current protein staining and de-staining protocols are tedious and time consuming, and therefore prolong the sample preparation time for mass spectrometry. Methodology and Principal Findings: We developed a 1-minute covalent pre-gel staining protocol for proteins, which does not require de-staining before the mass spectrometry analysis. We investigated the electrophoretic properties of derivatized proteins and peptides and studied their behavior in mass spectrometry. Further, we elucidated the preferred reaction of proteins with Uniblue A and demonstrate the integration of the peptide derivatization into typical informatics tools. Conclusions and Significance: The Uniblue A staining method drastically speeds up the sample preparation for the mass spectrometry based identification of proteins. The application of this chemo-proteomic strategy will be advantageous for routine quality control of proteins and for time-critical tasks in protein analysis.
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  • Mata-Gómez, M. A., Yasui, M. T., Guerrero-Rangel, A., Valdés-Rodríguez, S., & Winkler, R. (2012). Accelerated identification of proteins by mass spectrometry by employing covalent pre-gel staining with uniblue A. PloS One, 7 (2), e31438.
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  • description.provenance : Approved for entry into archive by Deborah Campbell(deborah.campbell@oregonstate.edu) on 2012-07-30T17:56:34Z (GMT) No. of bitstreams: 3 license_rdf: 22765 bytes, checksum: 56265f5776a16a05899187d30899c530 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) YasuiMatthewTChemicalBiologicalEnvironmentalEngineringAcceleratedIdentificationProteins.pdf: 352779 bytes, checksum: b8b65685c4eb927aed5388185e06d1d6 (MD5)
  • description.provenance : Made available in DSpace on 2012-07-30T17:56:34Z (GMT). No. of bitstreams: 3 license_rdf: 22765 bytes, checksum: 56265f5776a16a05899187d30899c530 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) YasuiMatthewTChemicalBiologicalEnvironmentalEngineringAcceleratedIdentificationProteins.pdf: 352779 bytes, checksum: b8b65685c4eb927aed5388185e06d1d6 (MD5) Previous issue date: 2012-02-17
  • description.provenance : Submitted by Deborah Campbell (deborah.campbell@oregonstate.edu) on 2012-07-30T17:53:48Z No. of bitstreams: 3 license_rdf: 22765 bytes, checksum: 56265f5776a16a05899187d30899c530 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) YasuiMatthewTChemicalBiologicalEnvironmentalEngineringAcceleratedIdentificationProteins.pdf: 352779 bytes, checksum: b8b65685c4eb927aed5388185e06d1d6 (MD5)

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