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MicroRNAs control neurobehavioral development and function in zebrafish

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https://ir.library.oregonstate.edu/concern/articles/6t053g883

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  • microRNAs (miRNAs) have emerged as regulators of a broad spectrum of neurodevelopmental processes, including brain morphogenesis, neuronal differentiation, and survival. While the role of miRNAs in establishing and maintaining the developing nervous system is widely appreciated, the developmental neurobehavioral role of miRNAs has yet to be defined. Here we show that transient disruption of brain morphogenesis by ethanol exposure results in behavioral hyperactivity in larval zebrafish challenged with changes in lighting conditions. Aberrations in swimming activity persist in juveniles that were developmentally exposed to ethanol. During early neurogenesis, multiple gene expression profiling studies revealed widespread changes in mRNA and miRNA abundance in ethanol-exposed embryos. Consistent with a role for miRNAs in neurobehavioral development, target prediction analyses identified multiple miRNAs misexpressed in the ethanol-exposed cohorts that were also predicted to target inversely expressed transcripts known to influence brain morphogenesis. In vivo knockdown of miR9/9* or miR-153c persistently phenocopied the effect of ethanol on larval and juvenile swimming behavior. Structural analyses performed on adults showed that repression of miR-153c during development impacts craniofacial skeletal development. Together, these data support an integral role for miRNAs in the establishment of vertebrate neurobehavioral and skeletal systems.-Tal, T. L., Franzosa, J. A., Tilton, S. C., Philbrick, K. A., Iwaniec, U. T., Turner, R. T., Waters, K. M., Tanguay, R. L. MicroRNAs control neurobehavioral development and function in zebrafish. FASEB J. 26, 1452-1461 (2012). www.fasebj.org
  • Keywords: Brain, Morphogenesis, Alcohol, Conditional loss, Osteoblast differentiation, Gene expression, Microarray data, Neural progenitors, Ethanol exposure, Larval zebrafish
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  • Tal, T. L., Franzosa, J. A., Tilton, S. C., Philbrick, K. A., Iwaniec, U. T., Turner, R. T., . . . . (2012). MicroRNAs control neurobehavioral development and function in zebrafish. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, 26(4), 1452-1461. doi: 10.1096/fj.11-194464
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  • 26
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  • 4
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  • This work was supported by U.S. National Institute of Environmental Health Sciences (NIEHS) Environmental Health Sciences Core Center grant ES00210, NIEHS training grant T32ES7060, an Oregon State University (OSU) Linus Pauling Institute grant to R.L.T., and Superfund Basic Research Program grant NIEHS P42 ES016465 to R.L.T. and K.M.W.
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