High frequency transformation of cottonwoods (genus Populus) by Agrobacterium rhizogene Public Deposited

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  • Many species of Populus, particularly cottonwoods of sections Aigeiros and Tacamahaca, remain recalcitrant to genetic transformation. We demonstrate that transgenic trees can be readily produced in several poplar genotypes using wild-type Agrobacterium rhizogenes. Hairy roots were produced in a variety of clones that included Populus trichocarpa Torr. & A. Gray, Populus deltoides Bartr. ex Marsh., P. trichocarpa x P. deltoides hybrids, and Populus alba L. x Populus grandidentata Michx., some of which were otherwise recalcitrant to regeneration or transformation. The frequency of hairy roots ranged from 17 to 92%; nearly all hairy roots could be regenerated into transgenic plants. Among 18 transgenic lines that were confirmed by Southern analysis and grown into small trees, 5 showed severe dwarfism and other phenotypic abnormalities, while the majority grew normally. Agrobacterium rhizogenes appears to be an effective means for transformation of diverse poplar genotypes and may be useful in genotypes where disarmed strains of Agrobacterium or regeneration protocols are inadequate to produce transgenic plants.
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  • Han, K.-H., M.P. Gordon, and S.H. Strauss. 1997. High frequency transformation of cottonwoods (genus Populus) by Agrobacterium rhizogenes. Canadian Journal of Forest Research 27(4): 464-470.
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