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  • Background: In Drosophila, male flies require the expression of the male-specific Fruitless protein (FRU[superscript M]) within the developing pupal and adult nervous system in order to produce male courtship and copulation behaviors. Recent evidence has shown that specific subsets of FRU[superscript M] neurons are necessary for particular steps of courtship and copulation. In these neurons, FRU[superscript M] function has been shown to be important for determining sex-specific neuronal characteristics, such as neurotransmitter profile and morphology. Results: We identified a small cohort of FRU[superscript M] interneurons in the brain and ventral nerve cord by their co-expression with the transcription factor Engrailed (En). We used an En-GAL4 driver to express a fru[superscript M] RNAi construct in order to selectively deplete FRU[superscript M] in these En/FRU[superscript M] co-expressing neurons. In courtship and copulation tests, these males performed male courtship at wild-type levels but were frequently sterile. Sterility was a behavioral phenotype as these En-fru[superscript M]RNAi males were less able to convert a copulation attempt into a stable copulation, or did not maintain copulation for long enough to transfer sperm and/or seminal fluid. Conclusions: We have identified a population of interneurons necessary for successful copulation in Drosophila. These data confirm a model in which subsets of FRU[superscript M] neurons participate in independent neuronal circuits necessary for individual steps of male behavior. In addition, we have determined that these neurons in wild-type males have homologues in females and fru mutants, with similar placement, projection patterns, and neurochemical profiles.
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  • description.provenance : Submitted by Deborah Campbell (deborah.campbell@oregonstate.edu) on 2013-07-24T18:19:50Z No. of bitstreams: 6 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) LathamKristinLZoologySmallCohortFRUM.pdf: 1785224 bytes, checksum: 65a787f7a9303901817d11485cffd6fb (MD5) LathamKristinLZoologySmallCohortFRUMfile1.pdf: 70707 bytes, checksum: 7a8b0feda76470911e3cea4d0140c67e (MD5) LathamKristinLZoologySmallCohortFRUMfile2.pdf: 68044 bytes, checksum: 38c676079cd4d4ad4b33f36b03d799de (MD5) LathamKristinLZoologySmallCohortFRUMfile3.pdf: 97706 bytes, checksum: 98f13307d94902b4017a3a0ace9050be (MD5) LathamKristinLZoologySmallCohortFRUMfile4.pdf: 6505 bytes, checksum: 23ddea5fb161bf4e8ad3a4372fcf5d9e (MD5)
  • description.provenance : Made available in DSpace on 2013-07-24T18:20:17Z (GMT). No. of bitstreams: 6 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) LathamKristinLZoologySmallCohortFRUM.pdf: 1785224 bytes, checksum: 65a787f7a9303901817d11485cffd6fb (MD5) LathamKristinLZoologySmallCohortFRUMfile1.pdf: 70707 bytes, checksum: 7a8b0feda76470911e3cea4d0140c67e (MD5) LathamKristinLZoologySmallCohortFRUMfile2.pdf: 68044 bytes, checksum: 38c676079cd4d4ad4b33f36b03d799de (MD5) LathamKristinLZoologySmallCohortFRUMfile3.pdf: 97706 bytes, checksum: 98f13307d94902b4017a3a0ace9050be (MD5) LathamKristinLZoologySmallCohortFRUMfile4.pdf: 6505 bytes, checksum: 23ddea5fb161bf4e8ad3a4372fcf5d9e (MD5) Previous issue date: 2013-05-21
  • description.provenance : Approved for entry into archive by Deborah Campbell(deborah.campbell@oregonstate.edu) on 2013-07-24T18:20:17Z (GMT) No. of bitstreams: 6 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) LathamKristinLZoologySmallCohortFRUM.pdf: 1785224 bytes, checksum: 65a787f7a9303901817d11485cffd6fb (MD5) LathamKristinLZoologySmallCohortFRUMfile1.pdf: 70707 bytes, checksum: 7a8b0feda76470911e3cea4d0140c67e (MD5) LathamKristinLZoologySmallCohortFRUMfile2.pdf: 68044 bytes, checksum: 38c676079cd4d4ad4b33f36b03d799de (MD5) LathamKristinLZoologySmallCohortFRUMfile3.pdf: 97706 bytes, checksum: 98f13307d94902b4017a3a0ace9050be (MD5) LathamKristinLZoologySmallCohortFRUMfile4.pdf: 6505 bytes, checksum: 23ddea5fb161bf4e8ad3a4372fcf5d9e (MD5)

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