Conifer DBMagic: a database housing multiple de novo transcriptome assemblies for 12 diverse conifer species Public Deposited

http://ir.library.oregonstate.edu/concern/defaults/9019s298d

This is the publisher’s final pdf. The published article is copyrighted by Springer and can be found at:  http://link.springer.com/journal/11295. To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work.

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Conifers comprise an ancient and widespread plant lineage of enormous commercial and ecological value. However, compared to model woody angiosperms, such as Populus and Eucalyptus, our understanding of conifers remains quite limited at a genomic level. Large genome sizes (10,000–40,000 Mbp) and large amounts of repetitive DNA have limited efforts to produce a conifer reference genome, and genomic resource development has focused primarily on characterization of expressed sequences. Here, we report the completion of a conifer transcriptome sequencing project undertaken in collaboration with the U.S. DOE Joint Genome Institute that resulted in production of almost 12 million sequence reads. Five loblolly pine (Pinus taeda) cDNA libraries representing multiple tissues, treatments, and genotypes produced over four million sequence reads that, along with available Sanger expressed sequence tags, were used to create contig assemblies using three different assembly algorithms: Newbler, MiraEST, and NGen. In addition, libraries from 11 other conifer species, as well as one member of the Gnetales (Gnetum gnemon), produced 0.4 to 1.2 million sequence reads each. Among the selected conifer species were representatives of each of the seven phylogenetic families in the Coniferales: Araucariaceae, Cephalotaxaceae, Cupressaceae, Pinaceae, Podocarpaceae, Sciadopityaceae, and Taxaceae. Transcriptome builds for each species were generated using each of the three assemblers. All contigs for every species generated using each assembler can be obtained from Conifer DBMagic, a public database for searching, viewing, and downloading contig sequences, the associated sequence reads, and their annotations.
Resource Type
DOI
Date Available
Date Issued
Citation
  • W Walter Lorenz, Savavanaraj Ayyampalayam, John M Bordeaux, Glenn T Howe, Kathleen D Jermstad, David B Neale, . . . . (2012). Conifer DBMagic: A database housing multiple de novo transcriptome assemblies for 12 diverse conifer species. Tree Genetics & Genomes, 8(6), 1477. doi: 10.1007/s11295-012-0547-y
Academic Affiliation
Series
Keyword
Rights Statement
Funding Statement (additional comments about funding)
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2012-12-26T22:49:47Z (GMT). No. of bitstreams: 5 HoweGlennForestryConiferDBMagicDatabaseSupplementaryFile1.xlsx: 18285 bytes, checksum: debe3fd7d6f55f32c375dbf1e249c96e (MD5) HoweGlennForestryConiferDBMagicDatabaseSupplementaryFile2.xlsx: 17491 bytes, checksum: 074ce5d0ce9fade650fa645d035a40ed (MD5) HoweGlennForestryConiferDBMagicDatabaseSupplementaryFile3.xlsx: 18735 bytes, checksum: 6f9f26a535761727cbdb42232fe287ac (MD5) HoweGlennForestryConiferDBMagicDatabaseSupplementaryFile4.pptx: 2101682 bytes, checksum: b2277172a383c3db38ed424a90d3aaee (MD5) HoweGlennForestryConiferDBMagicDatabase.pdf: 417127 bytes, checksum: 6f5ed3fbe2af1e5d6a2f8f5d51aa1a4a (MD5) Previous issue date: 2012-12
  • description.provenance : Approved for entry into archive by Deanne Bruner(deanne.bruner@oregonstate.edu) on 2012-12-26T22:49:47Z (GMT) No. of bitstreams: 5 HoweGlennForestryConiferDBMagicDatabaseSupplementaryFile1.xlsx: 18285 bytes, checksum: debe3fd7d6f55f32c375dbf1e249c96e (MD5) HoweGlennForestryConiferDBMagicDatabaseSupplementaryFile2.xlsx: 17491 bytes, checksum: 074ce5d0ce9fade650fa645d035a40ed (MD5) HoweGlennForestryConiferDBMagicDatabaseSupplementaryFile3.xlsx: 18735 bytes, checksum: 6f9f26a535761727cbdb42232fe287ac (MD5) HoweGlennForestryConiferDBMagicDatabaseSupplementaryFile4.pptx: 2101682 bytes, checksum: b2277172a383c3db38ed424a90d3aaee (MD5) HoweGlennForestryConiferDBMagicDatabase.pdf: 417127 bytes, checksum: 6f5ed3fbe2af1e5d6a2f8f5d51aa1a4a (MD5)
  • description.provenance : Submitted by Deanne Bruner (deanne.bruner@oregonstate.edu) on 2012-12-26T22:40:47Z No. of bitstreams: 5 HoweGlennForestryConiferDBMagicDatabaseSupplementaryFile1.xlsx: 18285 bytes, checksum: debe3fd7d6f55f32c375dbf1e249c96e (MD5) HoweGlennForestryConiferDBMagicDatabaseSupplementaryFile2.xlsx: 17491 bytes, checksum: 074ce5d0ce9fade650fa645d035a40ed (MD5) HoweGlennForestryConiferDBMagicDatabaseSupplementaryFile3.xlsx: 18735 bytes, checksum: 6f9f26a535761727cbdb42232fe287ac (MD5) HoweGlennForestryConiferDBMagicDatabaseSupplementaryFile4.pptx: 2101682 bytes, checksum: b2277172a383c3db38ed424a90d3aaee (MD5) HoweGlennForestryConiferDBMagicDatabase.pdf: 417127 bytes, checksum: 6f5ed3fbe2af1e5d6a2f8f5d51aa1a4a (MD5)

Relationships

Parents:

This work has no parents.

Last modified Default