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Using lasers to probe the transient light absorption by proteorhodopsin in marine bacterioplankton

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https://ir.library.oregonstate.edu/concern/articles/9c67wp33t

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Abstract
  • We constructed an experimental apparatus that used lasers to provide the probe beams for measuring the transient absorption kinetics of bacterioplankton that contain proteorhodopsin, a microbial protein that binds retinal and is analogous to animal rhodopsin. With this approach we were able to observe photocycles characteristic of functioning retinylidene ion pumps. Using light from lasers instead of broadband sources as transmittance probe beams can be advantageous when examining optically dense, highly scattering samples such as concentrated microbial cultures. Such a laser-based approach may prove useful in shipboard studies for identifying proteorhodopsin in whole cell suspensions concentrated from seawater.
  • This paper was published in Applied Optics and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/ao/home.cfm. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.
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  • Russell A. Desiderio, Samuel R. Laney, Ricardo M. Letelier, and Stephen J. Giovannoni, "Using lasers to probe the transient light absorption by proteorhodopsin in marine bacterioplankton," Applied Optics, 46, 7329-7336 (2007)
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  • 46
Journal Issue/Number
  • 29
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  • Partial support for this research was provided by T. J. Cowles.
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