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Biological-based control for spotted wing drosophila, Drosophila suzukii using RNAi technology with cost-effective dsRNA

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  • A method for large quantity dsRNA production was developed since a small amount of dsRNA synthesized in vitro with commercial kits are used for small scale bioassay evaluations. Developing a large-scale production system for dsRNA is critical for practical RNAi application, especially for non-transgenic RNAi-based control. However, large-scale analysis or field application using dsRNA could be limited by the cost and time of dsRNA synthesis. To solve this problem, we have just established a mass production system using a microbial-induced dsRNA production to increase the feasibility of RNAi application for spotted wing drosophila (SWD), Drosophila suzukii, control. The microbial-based dsRNA production system is a good strategy for producing large quantities of dsRNA that can later be used for feeding assays.
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  • This work was supported in part by a Northwest Center for Small Fruits Research Grant, and base funding from USDA ARS CRIS 2072-22000-040-00D
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