Article

 

DAPI direct counting underestimates bacterial abundances and average cell size compared to AO direct counting Público Deposited

Conteúdo disponível para baixar

Baixar PDF
https://ir.library.oregonstate.edu/concern/articles/bz60cz024

Descriptions

Attribute NameValues
Creator
Abstract
  • We compared estimates of bacterial abundance and of average bacterial cell size based on direct counts of bacterioplankton cells stained with acridine orange (AO) or 4’-6-diamidino-2-phenylidole (DAPI). Estimates of numbers mll–1 of bacteria stained with DAPI were on average 70% (range 57–82%) of bacterial counts made with AO as the stain. Cell volumes estimated from DAPI-stained cells were even less, on average 59% (range 34–80%) of those determined from measurement of AO-stained cells. The combination of lower DAPI values for both cell numbers and cell volumes resulted in overall underestimation of total bacterial biovolume liter–1. DAPI-determined total biovolumes ranged from 25 to 61% of biovolume values obtained via counts and measurements of AO-stained cells in the two data sets. These results can be ascribed to the differential staining of cell components by DAPI and AO. We recommend that DAPI staining be used with caution for estimating bacterial standing stock parameters.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Suzuki, Marcelino T., Sherr, Evelyn B., Sherr, Barry F., ( 1993), DAPI direct counting underestimates bacterial abundances and average cell size compared to AO direct counting, Limnology and Oceanography, 38, doi: 10.4319/lo.1993.38.7.1566.
Journal Title
Journal Volume
  • 38
Journal Issue/Number
  • 7
Academic Affiliation
Declaração de direitos
Funding Statement (additional comments about funding)
  • This work was supported by NSF grants OCE 88-16428 and OCE 88-23091 to B. and E. Sherr.
Publisher
Language
Replaces
ISSN
  • 0024-3590

Relações

Parents:

This work has no parents.

Itens