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  • Bacteriophage S-CRM01 has been isolated from a freshwater strain of Synechococcus and shown to be present in the upper Klamath River valley in northern California and Oregon. The genome of this lytic T4-like phage has a 178,563 bp circular genetic map with 297 predicted protein-coding genes and 33 tRNA genes that represent all 20 amino acid specificities. Analyses based on gene sequence and gene content indicate a close phylogenetic relationship to the “photosynthetic” marine cyanomyophages infecting Synechococcus and Prochlorococcus. Such relatedness suggests that freshwater and marine phages can draw on a common gene pool. The genome can be considered as being comprised of three regions. Region 1 is populated predominantly with structural genes, recognized as such by homology to other T4-like phages and by identification in a proteomic analysis of purified virions. Region 2 contains most of the genes with roles in replication, recombination, nucleotide metabolism and regulation of gene expression, as well as 5 of the 6 signature genes of the photosynthetic cyanomyophages (hli03, hsp20, mazG, phoH and psbA; cobS is present in Region 3). Much of Regions 1 and 2 are syntenous with marine cyanomyophage genomes, except that a segment encompassing Region 2 is inverted. Region 3 contains a high proportion (85%) of genes that are unique to S-CRM01, as well as most of the tRNA genes. Regions 1 and 2 contain many predicted late promoters, with a combination of CTAAATA and ATAAATA core sequences. Two predicted genes that are unusual in phage genomes are homologs of cellular spoT and nusG.
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  • description.provenance : Made available in DSpace on 2014-08-22T17:14:40Z (GMT). No. of bitstreams: 2 DreherTheoMicrobiologyFreshwaterCyanophageGenome.pdf: 434118 bytes, checksum: 506728574c5d4a3c06c9f2d72dd849ac (MD5) DreherTheoMicrobiologyFreshwaterCyanophageGenome_SupplementaryMaterial.zip: 387726 bytes, checksum: 410c2dee85d811f60f2551b601d13c6e (MD5) Previous issue date: 2011-05-23
  • description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2014-08-22T17:14:40Z (GMT) No. of bitstreams: 2 DreherTheoMicrobiologyFreshwaterCyanophageGenome.pdf: 434118 bytes, checksum: 506728574c5d4a3c06c9f2d72dd849ac (MD5) DreherTheoMicrobiologyFreshwaterCyanophageGenome_SupplementaryMaterial.zip: 387726 bytes, checksum: 410c2dee85d811f60f2551b601d13c6e (MD5)
  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2014-08-22T17:14:21Z No. of bitstreams: 2 DreherTheoMicrobiologyFreshwaterCyanophageGenome.pdf: 434118 bytes, checksum: 506728574c5d4a3c06c9f2d72dd849ac (MD5) DreherTheoMicrobiologyFreshwaterCyanophageGenome_SupplementaryMaterial.zip: 387726 bytes, checksum: 410c2dee85d811f60f2551b601d13c6e (MD5)

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