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Method development for extraction and purification of dermal RNA from FVB/N mice treated with environmental PAH mixtures Public Deposited

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  • Epidermal RNA samples from FVB/N mice treated with PAH standards and environmental PAH mixtures were harvested and purified using Trizol extraction and RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5), indicating significant RNA degradation and the need to modify handling, collection, and processing of skin to limit degradation. Potential sources of RNA degrdation include RNase activity, both endogenous and exogenous, thermal degradation during heat-induced epidermal-dermal seperation, and contamination of stock solutions. Testing of the modified method produced dermal RNA samples with acceptable RIN integrity levels (RIN mean 7.6). Variations of the modified method were performed and compared to one another for method optimization.
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  • description.provenance : Made available in DSpace on 2011-06-29T18:49:15Z (GMT). No. of bitstreams: 1 Larkin_BRR_finaldraft.pdf: 940478 bytes, checksum: efc559564eafe7d274b1ebf9320580de (MD5) Previous issue date: 2010
  • description.provenance : Submitted by Patricia Black (patricia.black@oregonstate.edu) on 2011-06-29T18:49:15Z No. of bitstreams: 1 Larkin_BRR_finaldraft.pdf: 940478 bytes, checksum: efc559564eafe7d274b1ebf9320580de (MD5)

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